Identification of nitrated proteins in the normal rat brain using a proteomics approach
| Autor: | Koichi Okamoto, Sachiyo Ichinose, Akira Omori, Yoko Suzuki, Makoto Tanaka, Makoto Sohmiya |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Proteomics Chaperonins Protein subunit Blotting Western Clathrin Pathogenesis Neurofilament Proteins Glial Fibrillary Acidic Protein medicine Animals Electrophoresis Gel Two-Dimensional Amino Acid Sequence Rats Wistar Amyotrophic lateral sclerosis Tyrosine Peptide sequence Chromatography High Pressure Liquid Brain Chemistry Cerebral Cortex Nitrates biology Glial fibrillary acidic protein Chemistry General Medicine medicine.disease Molecular biology Rats Neurology Biochemistry Clathrin Heavy Chains biology.protein Neurology (clinical) Chaperonin Containing TCP-1 |
| Zdroj: | Neurological Research. 27:630-633 |
| ISSN: | 1743-1328 0161-6412 |
| DOI: | 10.1179/016164105x22039 |
| Popis: | Background: The nitration of tyrosine has been suggested to play a role in the pathogenesis of neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD) and Alzheimer's disease (AD). Methods: In the present study, we identified four targets of protein nitration, T-complex polypeptide 1α subunit (TCP-1), neurofilament L (NFL), glial fibrillary acidic protein (GFAP) and clathrin heavy chain (CHC), in the normal rat cortex using a proteomics approach. Conclusions: There have been no reports on these proteins being identified by proteomics as nitrated forms in the brain. For further study, we have to investigate alterations in these nitrated proteins during aging and in neurodegenerative disorders. |
| Databáze: | OpenAIRE |
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