In vitro-constructed RP4-prime Plasmids Mediate Orientated Mobilization of the Proteus morganii Chromosome
| Autor: | Willem F. Coetzee, J. N. Coetzee, Yaffa Beck |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
DNA
Bacterial Genetic Markers Genetics DNA Recombinant Chromosome Mapping Chromosome Chromosomes Bacterial Biology Proteus Proteus morganii Microbiology Phenotype Molecular biology In vitro Homology (biology) law.invention Plasmid law Recombinant DNA Chromosomal dna Transformation Bacterial Plasmids |
| Zdroj: | Microbiology. 128:1163-1169 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-128-6-1163 |
| Popis: | Summary: RP4-prime plasmids containing inserts of Proteus morganii strain 2815 chromosomal DNA (0.5-13 megadaltons in size) were constructed in vitro. When introduced into the latter organism, the hybrid plasmids promoted transfer of chromosomal markers in an orientated manner, with recombinant frequenciEs From 10−4 to 10−6 per donor. Transconjugants expressed the tetracycline-resistant phenotype of the plasmids. Plasmid-guided trajectories overlapped and a circular map of chromosome markers was constructed. Correspondence between size of inserted chromosomal DNA in hybrid plasmids and numbers of markers transferred suggested that homology played a role in chromosome mobilization by these plasmids. |
| Databáze: | OpenAIRE |
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