Pulmonary fibrin microembolism with Echis carinatus venom in dogs: effects of a synthetic thrombin inhibitor
| Autor: | S M Chilton, Richard W. Carlson, R C Schaeffer, T. J. Hadden |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
medicine.medical_specialty
Physiology Viper Venoms Fibrinogen Fibrin Amino Acid Chloromethyl Ketones Leukocyte Count Dogs Thrombin Body Water Echis Prothrombinase Physiology (medical) Internal medicine medicine Animals Pulmonary wedge pressure Lung biology Platelet Count Respiration Hemodynamics biology.organism_classification Disease Models Animal medicine.anatomical_structure Endocrinology Echis carinatus Immunology Vascular resistance biology.protein Pulmonary Embolism medicine.drug |
| Zdroj: | Journal of Applied Physiology. 57:1824-1828 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/jappl.1984.57.6.1824 |
| Popis: | We produced pulmonary fibrin microembolism using an infusion of a prothrombin activator (Echis carinatus venom, 30 min, 0.5 NIH thrombin equivalent units/kg) in open-chest mongrel dogs. To determine the nonclotting effects of this venom on edemagenesis we infused an irreversible thrombin inhibitor, D-phenylalanyl-L-prolyl-L-arginine chloromethyl ketone (PPACK, 57 nmol X kg-1 X min-1 for 120 min), alone (n = 5) or with venom (Echis + PPACK, n = 5). The control group (n = 5) was given 1 ml of 0.9% NaCl. A decline in left atrial pressure (means +/- SE, 5.3 +/- 0.4 to 4.0 +/- 0.5 mmHg, P less than 0.05) and cardiac index (149 +/- 10 to 82 +/- 13 ml X min-1 X kg-1, P less than 0.01) in association with a marked increase in pulmonary arterial pressure (14.5 +/- 0.6 to 26.6 +/- 2.5 mmHg, P less than 0.001) and pulmonary vascular resistance (64 +/- 5 to 304 +/- 42 mmHg X ml-1 X min-1 X kg-1, P less than 0.001) was observed after 20 min of venom infusion. During this interval, pulmonary artery wedge pressure increased (4 +/- 1 to 12 +/- 4 mmHg, P less than 0.01) in four of eight animals. Fibrinogen declined below measurable levels and fibrin microemboli were seen in many pulmonary arterioles. These changes were not observed in the Echis + PPACK, PPACK, or control groups. Leukopenia and thrombocytopenia were observed in the Echis and Echis + PPACK groups.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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