Progressive Dendritic HCN Channelopathy during Epileptogenesis in the Rat Pilocarpine Model of Epilepsy
| Autor: | John W. Miller, Nicholas P. Poolos, Raimondo D'Ambrosio, Joaquin N. Lugo, Aaron H. Sheerin, Terrance D. Jones, Sangwook Jung, Anne E. Anderson |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Potassium Channels Cyclic Nucleotide-Gated Cation Channels Status epilepticus Epileptogenesis Article Rats Sprague-Dawley Epilepsy Channelopathy Internal medicine Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels medicine HCN channel Animals biology Chemistry General Neuroscience Pilocarpine Dendrites Hyperpolarization (biology) medicine.disease Rats Disease Models Animal Endocrinology Disease Progression biology.protein Channelopathies Phenobarbital medicine.symptom Neuroscience medicine.drug |
| Zdroj: | The Journal of Neuroscience. 27:13012-13021 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | Ion channelopathy plays an important role in human epilepsy with a genetic cause and has been hypothesized to occur in epilepsy after acquired insults to the CNS as well. Acquired alterations of ion channel function occur after induction of status epilepticus (SE) in animal models of epilepsy, but it is unclear how they correlate with the onset of spontaneous seizures. We examined the properties of hyperpolarization-activated cation (HCN) channels in CA1 hippocampal pyramidal neurons in conjunction with video-EEG (VEEG) recordings to monitor the development of spontaneous seizures in the rat pilocarpine model of epilepsy. Our results showed that dendritic HCN channels were significantly downregulated at an acute time point 1 week postpilocarpine, with loss of channel expression and hyperpolarization of voltage-dependent activation. This downregulation progressively increased when epilepsy was established in the chronic period. Surprisingly, VEEG recordings during the acute period showed that a substantial fraction of animals were already experiencing recurrent seizures. Suppression of these seizures with phenobarbital reversed the change in the voltage dependence ofIh, the current produced by HCN channels, but did not affect the loss of HCN channel expression. These results suggest two mechanisms of HCN channel downregulation after SE, one dependent on and one independent of recurrent seizures. This early and progressive downregulation of dendritic HCN channel function increases neuronal excitability and may be associated with both the process of epileptogenesis and maintenance of the epileptic state. |
| Databáze: | OpenAIRE |
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