The use of methanol extract of Leptospira interrogans in complement fixation tests for leptospirosis
| Autor: | Matilde Cota Koury, Cisalpino Eo, Rangel Hde A |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Leptospira
Serotype Antigens Bacterial biology Leptospira biflexa Methanol Complement Fixation Tests Immunology General Medicine biology.organism_classification Complement fixation test medicine.disease Applied Microbiology and Biotechnology Microbiology Leptospirosis Agglutination Tests Direct agglutination test Leptospiraceae Genetics medicine Humans Molecular Biology Leptospira interrogans |
| Zdroj: | Canadian Journal of Microbiology. 37:455-458 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m91-075 |
| Popis: | Methanol extracts were obtained from L. interrogans serovars icterohaemorrhagiae and canicola and L. biflexa serovar patoc. Human sera from 167 normal individuals and 40 patients with different infectious diseases tested by complement fixation tests showed negative reactions. Sera from 100 patients with a suspicion of leptospirosis were tested by complement fixation tests and microscopic agglutination reactions. Agreement of 84% was found for those two reactions. Positive microscopic agglutination tests at a dilution 1:20–1:400 with negative complement fixation tests were observed in 5% of patients and negative microscopic agglutination with complement fixation tests in the range of 1:20–1:1280 were observed in 11% of the cases. Key words: diagnostic, methanol extract, complement fixation test. |
| Databáze: | OpenAIRE |
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