Trophoblasts regulate natural killer cells via control of interleukin-15 receptor signaling
| Autor: | Sohyun Yun, Seo Y. Park, Byung Jun Ryu, Ae R. Han, Sung K. Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MAP Kinase Kinase 4 Receptor expression Immunology Biology Natural killer cell Cell Line 03 medical and health sciences Interleukin 21 Interferon-gamma 0302 clinical medicine medicine STAT5 Transcription Factor Immunology and Allergy Humans Extracellular Signal-Regulated MAP Kinases Lymphokine-activated killer cell Receptors Interleukin-15 Obstetrics and Gynecology Receptors Interleukin-2 Natural killer T cell Cell biology Trophoblasts Killer Cells Natural Interleukin-15 receptor 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Interleukin 15 Interleukin 12 K562 Cells T-Box Domain Proteins 030215 immunology Signal Transduction |
| Zdroj: | American journal of reproductive immunology (New York, N.Y. : 1989). 78(2) |
| ISSN: | 1600-0897 |
| Popis: | Problem Trophoblasts are known to decrease natural killer (NK) cell cytotoxicity. However, little is known about the interaction between trophoblasts and NK cells during pregnancy. Interleukin-15 (IL-15) is essential for priming NK cells and maximizing their effector functions. We investigated whether trophoblasts regulate NK cell activation via IL-15/IL-2 receptor and its signaling pathways. Method of study Natural killer-92 cells were primed with human first-trimester trophoblast cells (Sw.71) conditioned medium (CM) and co-cultured with K562 cells. Flow cytometry, Western blot analysis, and real-time PCR were performed to assess NK cell cytotoxicity, IL-15/IL-2 receptor expression, phosphorylation of STAT5 and MAPKs, and mRNA expression of IL-15-related genes. Results Natural killer-92 cells incubated with Sw.71 CM showed reduced cytotoxicity and IL-15-mediated proliferation, and expression of IL-15/IL-2 receptor subunits. STAT5 phosphorylation, EOMES and T-bet mRNA expressions, and ERK/JNK pathways of NK 92 cells were suppressed by Sw.71 CM. Productions of perforin, granzyme B, and IFN-γ were also downregulated. Conclusion Trophoblasts regulate human NK cell functions via suppression of IL-15/IL-2 receptor expression, transcription factors, and ERK/JNK pathways. |
| Databáze: | OpenAIRE |
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