B-lymphocyte heterogeneity: development and characterization of an alloantiserum which distinguishes B-lymphocyte differentiation alloantigens
| Autor: | Kenneth W. Sell, David H. Sachs, William E. Paul, Irwin Scher, Allan H Smith, Susan O. Sharrow, Aftab Ahmed |
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| Rok vydání: | 1977 |
| Předmět: |
Male
C57BL/6 Isoantigens Genetic Linkage Lymphocyte Immunology Receptors Antigen B-Cell Genes Recessive Mice Inbred Strains Spleen Immunoglobulin D Mice Antigen medicine Animals Immunology and Allergy Cytotoxic T cell Antiserum B-Lymphocytes Binding Sites Sex Chromosomes biology Cell Differentiation Complement System Proteins Articles Cytotoxicity Tests Immunologic biology.organism_classification Molecular biology medicine.anatomical_structure Immunoglobulin M Mice Inbred CBA biology.protein Female |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.145.1.101 |
| Popis: | CBA/N mice and F1 male mice, which are hemizygous for the CBA/N X chromosome, have an immune defect which is associated with the absence (deficiency) of a subpopulation of mature or late developing B lymphocytes. This characteristic was utilized to develop an antiserum that was specific for this subclass of B cells. C57BL/L mice were immunized with DBA/2 spleen calls, and the resulting antisera was absorbed with lymphoid cells derived from immunologically abnormal (CBA/N female X DBA/2 male)F1 male mice. The absorbed antisera was cytotoxic for a subpopulation of lymphocytes that was present in the spleens of adult DBA/2 and (CBA/N female X DBA/2 male)F1 female mice. The cells killed by the absorbed antisera were Ig-bearing, complement receptor-bearing B lymphocytes, which had a low-to-intermediate density of total surface Ig. Moreover, the cells remaining after treatment of adult (CBA/N female X DBA/2 male)F1 female spleen cells with the absorbed antisera and C had a high ratio of surface IgM to IgD. The development of this cytotoxic alloantisera, which is specific for a late developing (mature) subpopulation of B lymphocytes, will allow the functional characterization of subclasses of B lymphocytes. |
| Databáze: | OpenAIRE |
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