Identification of Active Transposon dTok , a Member of the hAT Family, in Rice
| Autor: | Ki-Hong Jung, Wenzhu Jiang, Mun-Hue Heu, Do-eun Lee, Sunok Moon, Dong Sun Lee, Gynheung An, Hak Soo Suh, Hee-Jong Koh |
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| Rok vydání: | 2006 |
| Předmět: |
Transposable element
Physiology Molecular Sequence Data Plant Science Biology Genes Plant Genome Chromosomes Plant P element Open Reading Frames Gene duplication Gene Alleles Phylogeny Transposase Repetitive Sequences Nucleic Acid Genetics Whole genome sequencing Polymorphism Genetic Base Sequence food and beverages Oryza Cell Biology General Medicine Mutagenesis Insertional Phenotype Composite transposon Multigene Family Mutation DNA Transposable Elements Genome Plant |
| Zdroj: | Plant and Cell Physiology. 47:1473-1483 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcl012 |
| Popis: | Recent completion of the sequencing of the rice genome has revealed that it contains >40% repetitive sequences, most of which are related to inactive transposable elements. During the molecular analysis of the floral organ number 1/multiple pistil 2 (fon1/mp2) mutant, we identified an active transposable element dTok0 that was inserted at the kinase domain of FON1, a homolog of CLAVATA1. Insertion of the element into FON1 generated an 8 bp duplication of its target sites, which is one of the major characteristics of the hAT family of transposons. The dTok0 element was actively transposed out of the FON1 gene, leaving 5-8 bp footprints. Reinsertion into a new location was observed at a low frequency. Analysis of the genome sequence showed that the rice cultivar 'Nipponbare' contains 25 copies of dTok elements; similar numbers were present in all the Oryza species examined. Because dTok0 does not encode a transposase, enzyme activity should be provided in trans. We identified a putative autonomous transposon, Tok1 that contains an intact open reading frame of the Ac-like transposase. |
| Databáze: | OpenAIRE |
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