Ultrasensitive, Multiplexed Detection of Cancer Biomarkers Directly in Serum by Using a Quantum Dot-Based Microfluidic Protein Chip
| Autor: | Shiping Song, Yao He, Lixing Weng, Juan Yan, Lianhui Wang, Chunhai Fan, Mei Hu, Haoting Lu |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Microfluidics Protein Array Analysis Color General Physics and Astronomy Nanotechnology Multiplexing Antibodies Quantum Dots Biomarkers Tumor medicine Animals Humans General Materials Science Protein chip Fluorescent Dyes Immunoassay medicine.diagnostic_test biology General Engineering Microfluidic Analytical Techniques Primary and secondary antibodies Fluorescence Carcinoembryonic Antigen Quantum dot Calibration biology.protein Cancer biomarkers alpha-Fetoproteins Blood Chemical Analysis |
| Zdroj: | ACS Nano. 4:488-494 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/nn901404h |
| Popis: | Sensitive and selective detection for cancer biomarkers are critical in cancer clinical diagnostics. Here we developed a microfluidic protein chip for an ultrasensitive and multiplexed assay of cancer biomarkers. Aqueous-phase-synthesized CdTe/CdS quantum dots (aqQDs) were employed as fluorescent signal amplifiers to improve the detection sensitivity. Secondary antibodies (goat anti-mouse IgG) were conjugated to luminescent CdTe/CdS QDs to realize a versatile fluorescent probe that could be used for multiplexed detection in both sandwich and reverse phase immunoassays. We found that our microfluidic protein chip not only possessed ultrahigh femtomolar sensitivity for cancer biomarkers, but was selective enough to be directly used in serum. This protein chip thus combines the high-throughput capabilities of a microfluidic network with the high sensitivity and multicolor imaging ability offered by highly fluorescent QDs, which can become a promising diagnostic tool in clinical applications. |
| Databáze: | OpenAIRE |
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