Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection
| Autor: | Chun-yu Jin, Aifu Lin, Li-Xin Xiang, Jian-Zhong Shao, Chong-bin Hu, Lu-lu Qin, Tong Shao, Dong-Dong Fan, Jian-fei Ji, Hao Li, Ning Su |
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| Rok vydání: | 2021 |
| Předmět: |
CD40
biology Germinal center hemic and immune systems chemical and pharmacologic phenomena Antibacterial Response Costimulatory regulation biochemical phenomena metabolism and nutrition Acquired immune system Immune system QL1-991 Immunoglobulin class switching Antibacterial immunity Immunity Immunology IgZ biology.protein bacteria CD154 Zoology |
| Zdroj: | Fish and Shellfish Immunology Reports, Vol 2, Iss, Pp 100038-(2021) |
| ISSN: | 2667-0119 |
| DOI: | 10.1016/j.fsirep.2021.100038 |
| Popis: | CD40 and CD154 are one of the best-characterized costimulatory molecules essential for adaptive immunity, which extensively involved in T and B cell activation, IgM Ab production, isotype class switching, germinal center formation and affinity maturation. However, the functionality of CD40 and CD154 in IgZ-mediated immunity remains limited. In this study, we explored the regulatory role of Cd40-Cd154 interaction in IgZ-mediated antibacterial immunity in zebrafish. The results showed that the IgZ-mediated antibacterial response can be significantly induced in response to A. hydrophila infection. The percentage of Cd40+IgZ+ B cells and the production of IgZ Ab were substantially increased upon A. hydrophila stimulation, but these reactions were markedly declined in Cd154 blockade fish by administering anti-Cd154 Ab or recombinant sCd40-Ig protein, accompanied with the impairment of the vaccine-initiated IgZ-mediated immunoprotection of fish against A. hydrophila infection. These observations suggested the essential role of Cd40-Cd154 interaction in IgZ-mediated bacterial immunity. Notably, the Cd40 and Cd154 costimulatory signals are required for a TD antigen-induced IgZ immunity, but are not indispensable for a TI antigen-induced IgZ immune response. These findings indicated the differential role of Cd40-Cd154 interaction in bacterial TD and TI antigen-induced IgZ immunity, which suggested the existence of diverse regulatory mechanisms underlying IgZ-mediated antibacterial immune reactions. To our knowledge, this is the first report to show the functional role of Cd40-Cd154 costimulatory signaling pathway in IgZ-mediated immune defense against bacterial infection. We hope this study will improve the current understanding of the coevolution between the IgZ/IgT immunoglobins and CD40/CD154 costimulatory molecules. |
| Databáze: | OpenAIRE |
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