An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

Autor: Willem G. Langenberg, Amitava Mitra, Roy French, Jan J. Rybczyński, Lingyu Zhang
Rok vydání: 2000
Předmět:
Zdroj: Plant Cell Reports. 19:241-250
ISSN: 1432-203X
0721-7714
Popis: A method for producing large numbers of transgenic wheat plants has been developed. With this approach, an average of 9.7% of immature embryo explants were transformed and generated multiple self- fertile, independently transformed plants. No untrans- formed plants, or escapes, were regenerated. This transformation procedure uses morphogenic calli derived from scutellum tissue of immature embryos of Triticum aestivum cv. Bobwhite co-bombarded with separate plasmids carrying a selectable marker gene (bar) and a gene of interest, respectively. Transformed wheat calli with a vigorous growth phenotype were obtained by extended culture on media containing 5.0 mg/l bialaphos. These calli retained morphogenic potential and were competent for plant regeneration for as long as 11 months. The bar gene and the gene of interest were co-expressed in T0 progeny plants. This wheat transformation protocol may facilitate quantita- tive production of multiple transgenic plants and signif- icantly reduce the cost and labor otherwise required for screening out untransformed escapes.
Databáze: OpenAIRE
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