DREAM regulates insulin promoter activity through newly identified DRE element
| Autor: | Teodora Daneva, Plamen Padeshki, Shina Pashova, Hristo Gagov, Radoslava Emilova, Volodia Georgiev |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
insulin
QH301-705.5 medicine.medical_treatment Repressor islets of langerhans promoter activity Biology General Biochemistry Genetics and Molecular Biology Transcription (biology) medicine Biology (General) General Immunology and Microbiology General Neuroscience Insulin Promoter Silencer Molecular biology humanities gene transcription medicine.anatomical_structure General Agricultural and Biological Sciences Pancreas Nucleus psychological phenomena and processes Intracellular dream |
| Zdroj: | Open Life Sciences, Vol 8, Iss 2, Pp 97-106 (2013) |
| ISSN: | 2391-5412 |
| Popis: | Downstream regulatory element antagonist modulator (DREAM) protein is a 31 kDa Ca2+-regulated transcriptional repressor. It functions as a silencer of the gene transcription. In low intracellular free Ca2+ concentration DREAM tightly binds to the downstream regulatory element (DRE) of gene promoter and impedes the transcription. In higher Ca2+ concentrations DREAM binds Ca2+ and disconnects from DRE of the gene promoter enabling transcription. We report that DREAM is expressed in different human tissues including the pancreas, where it is located in the islets of Langerhans. Location of DREAM in RIN-F5 cells in cultures is restricted to the nucleus and membranes and changes after increased Ca2+-levels. The proteins dissociate from dimmers to monomers and translocate out of the nucleus. The expression of DREAM in β-cells in the islets of Langerhans regulates the promoter activity of the insulin gene by directly interacting with the sequence located between +52 bp and +81 bp downstream of the transcriptional start site of the promoter. Our results provide evidence for the existence of DRE sequence in the insulin gene promoter. It is suggested that DREAM is a repressor of insulin gene transcription, whose effect is mediated by direct binding to DRE sequence. |
| Databáze: | OpenAIRE |
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