Elevated temperature increases meiotic crossover frequency via the interfering (Type I) pathway in Arabidopsis thaliana
| Autor: | Hong Ma, Hongkuan Wang, Gregory P. Copenhaver, Jiyue Huang, Yingxiang Wang, Scott M. Lewis, Alexander R. Bennett, Ashley R. Albright, Jennifer L. Modliszewski |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Arabidopsis Plant Science Interference (genetic) Biochemistry Chromosomal crossover Chromosome segregation Gene Expression Regulation Plant Plant Resistance to Abiotic Stress Chromosome Segregation Arabidopsis thaliana Cell Cycle and Cell Division Crossing Over Genetic Homologous Recombination Genetic Interference Genetics (clinical) Ecology biology Chromosome Biology Plant Anatomy Physics Temperature Eukaryota Classical Mechanics Plants Cell biology Nucleic acids Meiosis Phenotype Experimental Organism Systems Cell Processes Plant Physiology Physical Sciences Pollen Mechanical Stress Research Article lcsh:QH426-470 DNA recombination Arabidopsis Thaliana Brassica Research and Analysis Methods Chromosomes Plant 03 medical and health sciences Model Organisms Plant and Algal Models Plant-Environment Interactions Genetics Homologous chromosome Plant Defenses Molecular Biology Ecology Evolution Behavior and Systematics Plant Ecology Ecology and Environmental Sciences Organisms Biology and Life Sciences Cell Biology DNA Plant Pathology biology.organism_classification lcsh:Genetics Thermal Stresses 030104 developmental biology Mutation Homologous recombination |
| Zdroj: | PLoS Genetics PLoS Genetics, Vol 14, Iss 5, p e1007384 (2018) |
| ISSN: | 1553-7404 |
| DOI: | 10.1371/journal.pgen.1007384 |
| Popis: | For most eukaryotes, sexual reproduction is a fundamental process that requires meiosis. In turn, meiosis typically depends on a reciprocal exchange of DNA between each pair of homologous chromosomes, known as a crossover (CO), to ensure proper chromosome segregation. The frequency and distribution of COs are regulated by intrinsic and extrinsic environmental factors, but much more is known about the molecular mechanisms governing the former compared to the latter. Here we show that elevated temperature induces meiotic hyper-recombination in Arabidopsis thaliana and we use genetic analysis with mutants in different recombination pathways to demonstrate that the extra COs are derived from the major Type I interference sensitive pathway. We also show that heat-induced COs are not the result of an increase in DNA double-strand breaks and that the hyper-recombinant phenotype is likely specific to thermal stress rather than a more generalized stress response. Taken together, these findings provide initial mechanistic insight into how environmental cues modulate plant meiotic recombination and may also offer practical applications. Author summary Meiosis is the cell division used by sexually reproducing species to produce sperm and egg cells. During meiosis, programmed Double Strand Breaks (DSBs) occur on each chromosome, which allows DNA to be exchanged between chromosome pairs, resulting in crossovers (COs). COs are necessary to ensure faithful chromosome segregation during meiosis, and thus fertility, but are also an important source of genetic variation. As such, CO formation is tightly regulated. Despite this, CO frequency can be altered by external factors, such as temperature. In Arabidopsis thaliana, COs are formed through two pathways: interference-sensitive (Type I) and interference-insensitive (Type II). An increase in temperature results in an increase in CO frequency. Using a pollen based assay, we show that COs are formed in the Type I pathway, which accounts for approximately 85% of the COs in Arabidopsis. To investigate whether temperature-dependent COs are the result of additional DSBs, we used immunological staining to examine protein foci, which mark the sites of DSBs. We discovered that temperature likely increases CO frequency by shifting alternative repair outcomes, called non-crossovers, to favor additional COs, rather than by increasing DSBs. Lastly, we found that temperature is not a general stress response, as plants subject to salt stress did not exhibit an increase in CO frequency. Our results may prove valuable in aiding plant breeding by enhancing our ability to rapidly introgress suites of elite traits from wild-plants into their crop relatives, a method that is particularly attractive as it does not require genetic modifications. |
| Databáze: | OpenAIRE |
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