HPV16 CTL epitope peptide-activated dendritic cell and natural killer co-culture for therapy of cervical cancer in an animal model
| Autor: | Min Li, Qu-Xiao Du, Ji-Duo Shen, Feng-Tai Miao, Xiao-Hui Jia, Li Yao, Yan-Xia Hu |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Epidemiology Papillomavirus E7 Proteins Epitopes T-Lymphocyte Uterine Cervical Neoplasms Biology Cancer Vaccines Epitope Interleukin 21 Mice Tumor Cells Cultured Animals Humans Cytotoxicity Lymphokine-activated killer cell Public Health Environmental and Occupational Health Dendritic cell Dendritic Cells Natural killer T cell Flow Cytometry Coculture Techniques Peptide Fragments Killer Cells Natural Mice Inbred C57BL CTL Disease Models Animal Oncology Immunology Interleukin 12 Female Immunization T-Lymphocytes Cytotoxic |
| Zdroj: | Asian Pacific journal of cancer prevention : APJCP. 14(12) |
| ISSN: | 2476-762X |
| Popis: | There is increasing evidence that natural killer (NK) cells play an important role in antitumor immunity following dendritic cell (DC) vaccination. Little is known, however, about the optimal stimulation of DCs by epitopes and NK interactions for cytotoxicity in tumors. In this study, DC cells activated by the HPV16E7.49-57 epitope and LPS were co-cultured with NK cells in vitro, and then used ot immunize mice to study CTL activity of TC-1, which constitutively expresses HPV16E6E7, with an LDH release assay. Cytotoxicity in mice immunized with DC loaded with epitope HPVE7.49-57 vaccine co-cultured with NK was enhanced significantly ( p |
| Databáze: | OpenAIRE |
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