Ten years of external quality assessment (EQA) of Neisseria gonorrhoeae antimicrobial susceptibility testing in Europe elucidate high reliability of data
Autor: | Rachel Pitt, Peter Kohl, Catherine A Ison, Tania Crucitti, Michelle J Cole, Eszter Balla, Elizabeth Fagan, Susanne Buder, Martin Steinbakk, Guðrún Svanborg Hauksdóttir, Susanne Jacobsson, Sonja Pleininger, Blaženka Hunjak, Ineke Linde, Francesca Mifsud, Paola Stefanelli, Thea Bergheim, Sabrina Weiss, Eirini Siatravani, Anna Carannante, Samo Jeverica, Beata Młynarczyk-Bonikowska, Eva Tzelepi, Rosann Zammit Cassar, Magnus Unemo, Michaela Day, Maria-José Borrego, Gatis Pakarna, Jelena Viktorova, Julio A. Vázquez, Peter Pavlik, Raquel Abad, Gianfranco Spiteri, Lisa Rose, Steen Hoffmann, Jill Shepherd, Shila Seaton, Neil Woodford, Panayiota Maikanti, Nerteley Quaye, Angelika Stary |
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Přispěvatelé: | European Centre for Disease Prevention and Control (ECDC), European Centre for Disease Prevention and Control |
Rok vydání: | 2019 |
Předmět: |
Quality Control
0301 basic medicine Concordance 030106 microbiology Microbial Sensitivity Tests medicine.disease_cause lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Antibiotic resistance Euro-GASP Disk Diffusion Antimicrobial Tests Environmental health Drug Resistance Bacterial External quality assessment medicine media_common.cataloged_instance lcsh:RC109-216 Infecções Sexualmente Transmissíveis EUCAST 030212 general & internal medicine European union Etest media_common Resistência aos Antimicrobianos business.industry Reproducibility of Results European economic area (EEA) Neisseria gonorrhoeae Gonorrhoea Anti-Bacterial Agents Europe Infectious Diseases Data quality European Union (EU) Laboratories business Quality assurance Research Article |
Zdroj: | Repisalud Instituto de Salud Carlos III (ISCIII) BMC Infectious Diseases BMC Infectious Diseases, Vol 19, Iss 1, Pp 1-11 (2019) |
ISSN: | 1471-2334 |
Popis: | BACKGROUND: Confidence in any diagnostic and antimicrobial susceptibility testing data is provided by appropriate and regular quality assurance (QA) procedures. In Europe, the European Gonococcal Antimicrobial Susceptibility Programme (Euro-GASP) has been monitoring the antimicrobial susceptibility in Neisseria gonorrhoeae since 2004. Euro-GASP includes an external quality assessment (EQA) scheme as an essential component for a quality-assured laboratory-based surveillance programme. Participation in the EQA scheme enables any problems with the performed antimicrobial susceptibility testing to be identified and addressed, feeds into the curricula of laboratory training organised by the Euro-GASP network, and assesses the capacity of individual laboratories to detect emerging new, rare and increasing antimicrobial resistance phenotypes. Participant performance in the Euro-GASP EQA scheme over a 10 year period (2007 to 2016, no EQA in 2013) was evaluated. METHODS: Antimicrobial susceptibility category and MIC results from the first 5 years (2007-2011) of the Euro-GASP EQA were compared with the latter 5 years (2012-2016). These time periods were selected to assess the impact of the 2012 European Union case definitions for the reporting of antimicrobial susceptibility. RESULTS: Antimicrobial susceptibility category agreement in each year was ≥91%. Discrepancies in susceptibility categories were generally because the MICs for EQA panel isolates were on or very close to the susceptibility or resistance breakpoints. A high proportion of isolates tested over the 10 years were within one (≥90%) or two (≥97%) MIC log2 dilutions of the modal MIC, respectively. The most common method used was Etest on GC agar base. There was a shift to using breakpoints published by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) in the latter 5 years, however overall impact on the validity of results was limited, as the percentage categorical agreement and MIC concordance changed very little between the two five-year periods. CONCLUSIONS: The high level of comparability of results in this EQA scheme indicates that high quality data are produced by the Euro-GASP participants and gives confidence in susceptibility and resistance data generated by laboratories performing decentralised testing. The study was funded by the European Centre for Disease Prevention and Control (Framework Contract No. ECDC/2013/015). The funding body contributed to the design of the study, the interpretation of the data and to the writing of the manuscript. Sí |
Databáze: | OpenAIRE |
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