Apoptosis induced by butyrate is independent of Jak/STAT signaling in a fibrosarcoma cell line
| Autor: | Gustavo P. Amarante-Mendes, Cláudio A. Bonjardim, Catherine Ropert, Mariana Gontijo Ramos, Flávia L.A Rabelo, Jacqueline I. Alvarez-Leite, Gabriela Brumatti |
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| Rok vydání: | 2003 |
| Předmět: |
Fibrosarcoma
Caspase 2 Biophysics Apoptosis Caspase 3 Butyrate Biochemistry Tumor Cells Cultured medicine Humans Molecular Biology Enzyme Precursors Dose-Response Relationship Drug biology Janus kinase 1 Tumor Necrosis Factor-alpha Chemistry Janus Kinase 1 Cell Biology Protein-Tyrosine Kinases medicine.disease Cell biology DNA-Binding Proteins Enzyme Activation Butyrates STAT1 Transcription Factor Cell culture Caspases Trans-Activators biology.protein Tumor necrosis factor alpha |
| Zdroj: | Biochemical and Biophysical Research Communications. 301:968-973 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(03)00072-x |
| Popis: | The aim of this study was to evaluate the participation of the Jak-1 and STAT-1 proteins in sodium butyrate-induced apoptosis in 2C4 cells derived from human fibrosarcoma. Making use of Jak-1 or STAT-1 deficient cell lines, we demonstrated that the apoptotic process induced by butyrate is independent of the presence of these proteins. In addition, this work showed that, although the constitutive expression of pro-caspases-2 and -3 is reduced in STAT-1 cells, the activity of caspase-3 is preserved in both Jak-1 and STAT-1 deficient cells and is similar to that seen in 2C4 parental cells. In conclusion, we demonstrated that the absence of functionally active Jak-1 or STAT-1 protein directly affects the TNF-alpha-induced apoptosis, but does not alter the sodium butyrate-induced apoptosis in cells derived from human fibrosarcoma. |
| Databáze: | OpenAIRE |
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