Pre-conditioned mesenchymal stem cells ameliorate renal ischemic injury in rats by augmented survival and engraftment

Autor: Sheikh Riazuddin, Muhammad Afzal, Azra Mehmood, Sanam Saiqa Anwar, Muhammad Shareef Masoud, Shaheen N. Khan
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Pre-conditioning
Male
Pathology
lcsh:Medicine
MSCs
Pharmacology
Kidney
Kidney Function Tests
Rats
Sprague-Dawley
chemistry.chemical_compound
Ischemia
Medicine(all)
Acute kidney injury
Snap
Renal ischemia
General Medicine
SNAP
Acute Kidney Injury
medicine.anatomical_structure
Kidney Tubules
Cytoprotective factors
Stem cell
medicine.medical_specialty
Cell Survival
Neovascularization
Physiologic
S-Nitroso-N-Acetylpenicillamine
Mesenchymal Stem Cell Transplantation
Nitric Oxide
General Biochemistry
Genetics and Molecular Biology
medicine
Animals
Cell Proliferation
business.industry
Biochemistry
Genetics and Molecular Biology(all)
Research
Mesenchymal stem cell
lcsh:R
Mesenchymal Stem Cells
medicine.disease
Fibrosis
Culture Media
Rats
chemistry
Gene Expression Regulation
Cytoprotection
S-Nitroso-N-acetylpenicillamine
business
Zdroj: Journal of Translational Medicine
Journal of Translational Medicine, Vol 10, Iss 1, p 243 (2012)
ISSN: 1479-5876
Popis: Background Ischemia is the major cause of acute kidney injury (AKI), associated with high mortality and morbidity. Mesenchymal stem cells (MSCs) have multilineage differentiation potential and can be a potent therapeutic option for the cure of AKI. Methods MSCs were cultured in four groups SNAP (S-nitroso N-acetyl penicillamine), SNAP + Methylene Blue (MB), MB and a control for in vitro analysis. Cultured MSCs were pre-conditioned with either SNAP (100 μM) or MB (1 μM) or both for 6 hours. Renal ischemia was induced in four groups (as in in vitro study) of rats by clamping the left renal padicle for 45 minutes and then different pre-conditioned stem cells were transplanted. Results We report that pre-conditioning of MSCs with SNAP enhances their proliferation, survival and engraftment in ischemic kidney. Rat MSCs pre-conditioned with SNAP decreased cell apoptosis and increased proliferation and cytoprotective genes’ expression in vitro. Our in vivo data showed enhanced survival and engraftment, proliferation, reduction in fibrosis, significant improvement in renal function and higher expression of pro-survival and pro-angiogenic factors in ischemic renal tissue in SNAP pre-conditioned group of animals. Cytoprotective effects of SNAP pre-conditioning were abrogated by MB, an inhibitor of nitric oxide synthase (NOS) and guanylate cyclase. Conclusion The results of these studies demonstrate that SNAP pre-conditioning might be useful to enhance therapeutic potential of MSCs in attenuating renal ischemia reperfusion injury.
Databáze: OpenAIRE
Externí odkaz: