Nuclear envelope remodelling during human spermiogenesis involves somatic B-type lamins and a spermatid-specific B3 lamin isoform
| Autor: | Razan A. Elkhatib, Catherine Metzler-Guillemain, Michael J. Mitchell, J.M. Grillo, Nicolas Lévy, Vincent Achard, Marine Paci, Guy Longepied |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male congenital hereditary and neonatal diseases and abnormalities endocrine system Embryology animal structures Spermiogenesis Somatic cell Biology Mice Genetics medicine Animals Humans Protein Isoforms Inner membrane RNA Messenger Spermatogenesis Molecular Biology Nuclear Lamina Lamin Type B integumentary system Spermatid Obstetrics and Gynecology Cell Differentiation Spermatid differentiation Cell Biology Lamin Type A Spermatids Cell biology Chromatin medicine.anatomical_structure Gene Expression Regulation Reproductive Medicine embryonic structures Nuclear lamina Acrosome Lamin HeLa Cells Developmental Biology |
| Zdroj: | MHR: Basic science of reproductive medicine. 21:225-236 |
| ISSN: | 1460-2407 1360-9947 |
| Popis: | The nuclear lamina (NL) is a filamentous protein meshwork, composed essentially of lamins, situated between the inner nuclear membrane and the chromatin. There is mounting evidence that the NL plays a role in spermatid differentiation during spermiogenesis. The mouse spermatid NL is composed of the ubiquitous lamin B1 and the spermatid-specific lamin B3, an N-terminally truncated isoform of lamin B2. However, nothing is known about the NL in human spermatids. We therefore investigated the expression pattern and localization of A-type lamins (A, C and C2) and B-type lamins (B1, B2 and B3) during human spermiogenesis. Here, we show that a lamin B3 transcript is present in human spermatids and that B-type lamins are the only lamins detectable in human spermatids. We determine that, as shown for their mouse counterparts, human lamin B3, but not lamin B2, induces strong nuclear deformation, when ectopically expressed in HeLa cells. Co-immunofluorescence revealed that, in human spermatids, B-type lamins are present at the nuclear periphery, except in the region covered by the acrosome, and that as the spermatid matures the B-type lamins recede towards the posterior pole. Only lamin B1 remains detectable on 33-47% of ejaculated spermatozoa. On spermatozoa selected for normal head density, however, this fell to |
| Databáze: | OpenAIRE |
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