Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus Paracoccidioides

Autor: Julhiany de Fátima da Silva, Gisela Ferreira, Adriele Dandara Levorato Vinche, Rinaldo Poncio Mendes, Daniela Vanessa Moris de Oliveira, Ricardo de Souza Cavalcante, Camila Marçon, Alma Rosa González-Esquinca, Hans Garcia Garces, Iván De-la-Cruz-Chacón, Daniela Carvalho dos Santos
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidad de Ciencias y Artes de Chiapas, Universidade do Oeste Paulista (Unoeste)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Venomous Animals and Toxins including Tropical Diseases v.26 2020
The Journal of venomous animals and toxins including tropical diseases
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Scopus
Repositório Institucional da UNESP
The Journal of Venomous Animals and Toxins Including Tropical Diseases
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020)
Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 26, Article number: e20200023, Published: 28 OCT 2020
ISSN: 1678-9199
Popis: Made available in DSpace on 2021-06-25T10:46:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-28. Added 1 bitstream(s) on 2021-07-15T15:23:18Z : No. of bitstreams: 1 S1678-91992020000100337.pdf: 7061245 bytes, checksum: f1175c6ed210c2f03ef23c270bd20f59 (MD5) Background: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or noninfectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides. Methods: The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy. Results: Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 μg.mL-1); on yeasts of Candida spp. (MIC of 125 to 250 μg.mL-1), including C. krusei (250 μg.mL-1), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 μg.mL-1). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18). Conclusion: Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications. Department of Tropical Disease and Imaging Diagnosis Botucatu Medical School São Paulo State University (UNESP) Universidad de Ciencias y Artes de Chiapas Institute of Biosciences of Botucatu São Paulo State University (UNESP) Faculdade de Ciências da Saúde Universidade do Oeste Paulista (Unoeste) Department of Tropical Disease and Imaging Diagnosis Botucatu Medical School São Paulo State University (UNESP) Institute of Biosciences of Botucatu São Paulo State University (UNESP)
Databáze: OpenAIRE
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