Critical roles for the digestive vacuole plasmepsins ofPlasmodium falciparumin vacuolar function
| Autor: | Tonya D. Bonilla, Hisashi Fujioka, John B. Dame, Charles A. Yowell, J. Alfredo Bonilla |
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| Rok vydání: | 2007 |
| Předmět: |
Erythrocytes
Endosome Plasmodium falciparum Mutant Protozoan Proteins Plasmepsin Vacuole Microbiology Apicomplexa Antimalarials Parasitic Sensitivity Tests Western blot medicine Animals Aspartic Acid Endopeptidases Molecular Biology chemistry.chemical_classification medicine.diagnostic_test biology HIV Protease Inhibitors biology.organism_classification Cell biology Microscopy Electron Enzyme chemistry Biochemistry Vacuoles Gene Deletion |
| Zdroj: | Molecular Microbiology. 65:64-75 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.2007.05768.x |
| Popis: | Knockout mutants of Plasmodium falciparum lacking pfpm1, pfpm2 and pfhap (triple-PM KO), and mutants lacking all four digestive vacuole (DV) plasmepsins (pfpm4, pfpm1, pfpm2 and pfhap; quadruple-PM KO), were prepared by double cross-over integration effecting chromosomal deletions of up to 14.6 kb. The triple-PM KO was similar to the parental line (3D7) in growth rate, morphology and sensitivity to proteinase inhibitors. The quadruple-PM KO showed a significantly slower rate of growth in standard medium, which manifested as delayed schizont maturation accompanied by reduced formation of haemozoin. In amino acid-limited medium, the reduction in growth rate of the quadruple-PM KO was pronounced. The sensitivity of both the triple- and quadruple-PM KOs to six different HIV aspartic proteinase inhibitors was comparable to that of 3D7, thus establishing that the DV plasmepsins were not the primary targets of the antimalarial activity of these clinically important compounds. Electron microscopic analysis revealed the presence of multilamellar bodies resembling ceroid in the DV of the quadruple-PM KO, and intermediates of the autophagic pathway accumulated as determined by Western blot analysis. Thus, the DV plasmepsins, although not essential, contribute significantly to the fitness of the parasite and are required for efficient degradation of endosomal vesicles delivered to the DV. |
| Databáze: | OpenAIRE |
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