Functional circuitry of neuro-immune communication in the mesenteric lymph node and spleen

Autor: Kaitlin Murray, Colin Reardon, Mariana Barboza Gardner, Ingrid Brust-Mascher, Kavi M. Rude
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Neuroimmunology
Inbred Strains
Stimulation
Behavioral Neuroscience
Mice
Norepinephrine
0302 clinical medicine
Cholinergic anti-inflammatory pathway
Receptors
Abdomen
Psychology
Mesenteric lymph nodes
medicine.anatomical_structure
Adrenergic
Peripheral nervous system
Female
Mesenteric Artery
Acetylcholine
medicine.drug
Vagus Nerve Stimulation
Neuroimmunomodulation
Peripheral optogenetics
Immunology
Spleen
Mice
Inbred Strains
beta-1
vagal nerve stimulation
03 medical and health sciences
Mesenteric Artery
Superior
Superior
Superior mesenteric ganglion
medicine
Animals
Neurology & Neurosurgery
Endocrine and Autonomic Systems
business.industry
Neurosciences
030104 developmental biology
Cancer research
Cholinergic
Lymph Nodes
Receptors
Adrenergic
beta-1
business
030217 neurology & neurosurgery
Zdroj: Brain, behavior, and immunity. 82
ISSN: 1090-2139
Popis: The peripheral nervous system is an active participant in immune responses capable of blocking aberrant activation of a variety of immune cells. As one of these neuro-immune circuits, the cholinergic anti-inflammatory pathway has been well established to reduce the severity of several immunopathologies. While the activation of this pathway by vagal nerve stimulation requires sympathetic innervation of the spleen, the neuro-immune circuitry remains highly controversial. Neuro-immune pathways in other lymphoid tissues such as mesenteric lymph nodes (MLN) that are critical to the surveillance of the small intestine and proximal colon have not been assessed. Using conditionally expressed Channelrhodopsin, selective stimulation of sympathetic post-ganglionic neurons in the superior mesenteric ganglion (SMG) prevented macrophage activation and LPS-induced TNFα production in the spleen and MLN, but not in the inguinal LN. Site selective stimulation of the SMG induced the release of norepinephrine, resulting in β2AR dependent acetylcholine release in the MLN and spleen. VNS-evoked release of norepinephrine and acetylcholine in the MLN and spleen was significantly reduced using selective optogenetic blockade applied at the SMG. Additionally, this optogenetic blockade restored LPS-induced TNFα production, despite VNS. These studies identify the superior mesenteric ganglion as a critical node in a neuro-immune circuit that can inhibit immune function in the MLN and the spleen.
Databáze: OpenAIRE
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