Effects and potential mechanisms of rapamycin on MPTP-induced acute Parkinson’s disease in mice
| Autor: | Xie Yu, Xuemei Chen, Lishi Yin, Ming Wang, Hai-yan Luo, Zhenchun Luo, Yanping He, Fengwei Tian, Guifang Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Parkinson's disease medicine.medical_treatment Intraperitoneal injection Substantia nigra Striatum Pharmacology Pathogenesis Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Animals Medicine PI3K/AKT/mTOR pathway Sirolimus Advanced and Specialized Nursing business.industry MPTP Parkinson Disease medicine.disease Mice Inbred C57BL Substantia Nigra 030104 developmental biology Anesthesiology and Pain Medicine nervous system chemistry 1-Methyl-4-phenyl-1 2 3 6-tetrahydropyridine 030220 oncology & carcinogenesis Signal transduction business |
| Zdroj: | Annals of Palliative Medicine. 10:2889-2897 |
| ISSN: | 2224-5839 2224-5820 |
| Popis: | BACKGROUND Parkinson's disease is the second major neurodegenerative diseases secondarily to Alzheimer's disease. Rapamycin is a fermentation product, which derived from Streptomyces hygroscopius. The aim of this study is to investigate the effect of rapamycin and its potential mechanisms on the acute attack of 1-methyl-4-phenyl-1,2,3,6-four hydrogen pyridine (MPTP) induced Parkinson's disease (PD) in mice. METHODS PD model was established by intraperitoneal injection of MPTP for 5 days. The effect of intraperitoneal injection of rapamycin for treating the symptoms caused by PD was evaluated by behavior observation and HE pathological section. In order to understand the possible mechanism, immunofluorescence and immune precipitation mainly analyzes were used to measure the expression of critical protein p-4ebp1 in mammalian target of rapamycin (mTOR) signaling pathways in the striatum and substantia nigra. RESULTS Rapamycin can effectively alleviate symptoms of PD. The levels of key protein p-4EBP1 in the striatum and substantia nigra were both significantly higher in PD group compared with control group (P |
| Databáze: | OpenAIRE |
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