A holidic medium for Drosophila melanogaster
| Autor: | Fiona Kerr, Corinna Hopfen, Christine Niemeyer, Eric Blanc, Matthew P. Hoddinott, Carlos Ribeiro, Ricardo Leitão-Gonçalves, Scott D. Pletcher, Xiaoli He, Linda Partridge, Nancy J. Linford, Matthew D.W. Piper, George A Soultoukis, Mingyao Yang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Time Factors
Animal feed media_common.quotation_subject Longevity Genetics Behavioral Choice Behavior Biochemistry Egg laying Article 03 medical and health sciences Drug Delivery Systems 0302 clinical medicine Feeding behavior Animals Food science Amino Acids Molecular Biology Drosophila 030304 developmental biology media_common Genetics 0303 health sciences Behavior Animal biology Gene Expression Profiling Feeding Behavior Cell Biology biology.organism_classification Fecundity Animal Feed Drosophila melanogaster Fertility Folic acid Female 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Nat Methods NATURE METHODS Nature methods |
| Popis: | A critical requirement for research using model organisms is an appropriate, well-defined and consistent diet. There is currently no complete chemically defined (holidic) diet available for Drosophila melanogaster. We describe a holidic medium that is equal in performance to an oligidic diet optimized for adult fecundity and lifespan. It is also sufficient to support development over multiple generations, but at a reduced rate. During seven years of experiments, the holidic diet yielded more consistent experimental outcomes than oligidic food for adult fitness traits. Furthermore, nutrients and drugs are more accessible to flies in holidic medium and, similar to dietary restriction on oligidic food, amino acid dilution increases fly lifespan. We also report amino acid specific effects on food choice behavior and that folic acid from the microbiota is sufficient for development. These insights could not be gained using oligidic or meridic diets. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|