Humoral and cellular immune response to aMicrosporumcanisrecombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
| Autor: | Michel Monod, F. Brouta, S. Vermout, Bernard Mignon, Bertrand Losson, Frédéric Descamps |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Animal Experimentation
Cellular immunity Antigens Fungal Lymphocyte Guinea Pigs Enzyme-Linked Immunosorbent Assay Microbiology Immune system Antigen medicine Animals Dermatomycoses Microsporum Microsporum canis Antibodies Fungal Immunity Cellular biology General Medicine Acquired immune system biology.organism_classification Fungal antigen Virology Recombinant Proteins Infectious Diseases medicine.anatomical_structure Antibody Formation Humoral immunity Metalloproteases Peptide Hydrolases |
| Zdroj: | Medical Mycology. 41:495-501 |
| ISSN: | 1460-2709 1369-3786 |
| DOI: | 10.1080/13693780310001615385 |
| Popis: | In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|