Phylogenetic and cluster analysis of human rhinovirus species A (HRV-A) isolated from children with acute respiratory infections in Yamagata, Japan
| Autor: | Masatsugu Obuchi, Katsumi Mizuta, Hirokazu Kimura, Tadayuki Ahiko, Asumi Hirata, Nobuhiko Okabe, Hiroyuki Tsukagoshi, Tsutomu Itagaki, Masato Tashiro, Masahiro Noda, Yukio Morita, Asuka Suto, Yoko Aoki, Miho Akiyama |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Cancer Research Adolescent Rhinovirus Molecular Sequence Data Prevalence Biology medicine.disease_cause Japan Phylogenetics Virology Sequence Homology Nucleic Acid medicine Cluster Analysis Humans Respiratory system Child Respiratory Tract Infections Phylogeny Viral Structural Proteins Molecular Epidemiology Picornaviridae Infections Molecular epidemiology Phylogenetic tree Sequence Homology Amino Acid Respiratory infection Infant Sequence Analysis DNA medicine.disease Infectious Diseases Bronchiolitis Child Preschool RNA Viral Female |
| Zdroj: | Virus research. 147(2) |
| ISSN: | 1872-7492 |
| Popis: | We performed phylogenetic and cluster analysis of human rhinovirus species A (HRV-A) isolated from 76 children with acute respiratory infection in Yamagata prefecture, Japan during the period 2003–2007. Phylogenetic trees based on the nucleotide and amino acid sequences of the VP4/VP2 coding region showed that the present strains could be classified into 11 and 8 clusters, respectively. The homology among the present strains ranged from 66.6% to 100% at the nucleotide level and 84.7% to 100% at the amino acid level. The interspecies distance (mean ± standard deviation) was calculated to be 0.235 ± 0.048 at the nucleotide level and 0.076 ± 0.033 at the amino acid level. In addition, the phylogenetic trees created based on the nucleotide and amino acid sequences showed that HRV-A strains belonging to some clusters were associated with both upper respiratory infection and wheezy bronchiolitis, while other strains were associated with upper respiratory infection alone. These results suggest that the present HRV-A isolates had a wide nucleotide divergence and were associated with acute respiratory infection, including upper respiratory infection and wheezy bronchiolitis, in Yamagata prefecture, Japan during the investigation period. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect