Polyoma Viral DNA Replicated as a Nucleoprotein Complex in Close Association with the Host Cell Chromatin
| Autor: | Thomas Seebeck, Roger Weil |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
DNA Replication
viruses Immunology Eukaryotic DNA replication Kidney Tritium Microbiology Mice Viral Proteins chemistry.chemical_compound Nucleic acid thermodynamics Virology Animal Viruses Centrifugation Density Gradient Methods Animals Carbon Radioisotopes Cells Cultured biology DNA replication Nucleic Acid Hybridization Molecular biology Chromatin Nucleoprotein Nucleoproteins Histone chemistry Insect Science DNA Viral biology.protein Autoradiography Origin recognition complex Polyomavirus DNA Thymidine |
| Zdroj: | Journal of Virology. 13:567-576 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.13.3.567-576.1974 |
| Popis: | Polyoma viral DNA is shown to be replicated in close association with the mouse cell chromatin. Two virus-specific nucleoprotein complexes, designated complex A and B, can be dissociated from the isolated chromatin by gentle homogenization in 0.5 M NaCl. Complex A contains only replicating polyoma (Py) DNA whereas complex B contains only mature Py DNA I. The results show, furthermore, that complex A, containing viral DNA in different stages of replication, and complex B are both nucleoproteins with the same buoyant density. The data presently available suggest that newly synthesized stretches of Py DNA are immediately complexed with mouse cell histones and that complex B becomes the “core” of progeny Py virions. These results suggested that Py-induced replication of the mouse cell chromatin may be necessary to provide replicating Py DNA with histones. |
| Databáze: | OpenAIRE |
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