Effects of AFP-activated PI3K/Akt signaling pathway on cell proliferation of liver cancer
| Autor: | Xiaobing Huang, Ping Liang, Lu Zheng, Wei Gong, Jing Li, Ke Qiang Han, Yuming Li, Nan You |
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| Rok vydání: | 2013 |
| Předmět: |
Cell growth
Akt/PKB signaling pathway Liver Neoplasms General Medicine Hep G2 Cells Biology medicine.disease Molecular biology digestive system diseases Phosphatidylinositol 3-Kinases Real-time polymerase chain reaction Hepatocellular carcinoma medicine Humans RNA Messenger alpha-Fetoproteins Signal transduction Liver cancer Protein kinase B Proto-Oncogene Proteins c-akt PI3K/AKT/mTOR pathway Cell Proliferation Signal Transduction |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 35(5) |
| ISSN: | 1423-0380 |
| Popis: | This study aims to investigate effects of alpha-fetoprotein (AFP)-activated phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway on hepatocellular carcinoma cell proliferation. Active cirrhosis patients after hepatitis B infection (n = 20) and viral hepatitis patients with hepatocellular carcinoma (HCC) (n = 20) were selected as the subjects of the present study. Another 20 healthy subjects were selected as the control group. The serum AFP expression and liver tissue PI3K and Akt gene mRNA expression were detected. The hepatoma cell model HepG2 which had a stable expression of AFP gene was used. Real-time quantitative PCR and Western blot and other methods were used to analyze the intracellular PI3K and Akt protein levels. Compared with control group and cirrhosis group, the serum AFP levels in HCC group significantly increased, and the tissue PI3K and Akt mRNA expression also significantly increased. HepG2 cells were intervened using AFP, in which the PIK and Akt protein expression significantly increased. After intervention by use of AFP monoclonal antibodies or LY294002 inhibitor, the PIK and Akt protein expression in HepG2 cell was significantly decreased (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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