Characterization and functional analysis of novel circulating NK cell sub-populations
| Autor: | Nuchjira Takheaw, Supansa Pata, Surasakdi Wongratanacheewin, Nuttapol Chruewkamlow, Kodchakorn Mahasongkram, Kantinan Chuensirikulchai, Saichit Khummuang, Nobuo Sugiura, Witida Laopajon, Hideto Watanabe, Hiroaki Tateno, Ludthawun Kamuthachad, Watchara Kasinrerk |
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| Rok vydání: | 2019 |
| Předmět: |
Mice
Inbred BALB C Chemistry medicine.drug_class Immunology Innate lymphoid cell Antibodies Monoclonal General Medicine Monoclonal antibody Epitope Cell Line Natural killer cell Cell biology Killer Cells Natural Cell therapy Mice Cytolysis medicine.anatomical_structure medicine Animals Humans Immunology and Allergy Neural cell adhesion molecule K562 cells |
| Zdroj: | International Immunology. 31:515-530 |
| ISSN: | 1460-2377 |
| DOI: | 10.1093/intimm/dxz027 |
| Popis: | Natural killer (NK) cells are innate lymphoid cells having potent cytolytic function that provide host defense against microbial infections and tumors. Using our generated monoclonal antibody (mAb), named FE-1H10, new NK cell sub-populations in peripheral blood were identified. The molecules recognized by mAb FE-1H10 were expressed on a sub-population of CD3−CD56dim NK cells. The epitope recognized by mAb FE-1H10 was demonstrated to be N-glycan and proven to be different from CD57. Upon K562 stimulation, the CD56dimFE-1H10+ NK cell sub-population exhibited significantly lower cytolytic function with low ability to degranulate and release cytolytic granules compared to the CD56dimFE-1H10− NK cell sub-population. Moreover, the CD56dimFE-1H10+ NK cells produced less IFN-γ and TNF-α than the CD56dimFE-1H10− NK cells. We demonstrated here that mAb FE-1H10 could identify two sub-populations of circulating CD56dim NK cells with different functions. Our discovery of new sub-populations of NK cells improves our understanding of NK cell biology and may lead to the development of new approaches for NK cell therapy. |
| Databáze: | OpenAIRE |
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