Structural characterization and biological function of bivalent binding of CD2AP to intrinsically disordered domain of chikungunya virus nsP3 protein
Autor: | Vladislav Yu. Orekhov, Tetyana Lukash, Yulia Pustovalova, Francisco Dominguez, Peter Agback, Nikita Shiliaev, Tatiana Agback, Elena I. Frolova, Ilya Frolov |
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Rok vydání: | 2019 |
Předmět: |
Magnetic Resonance Spectroscopy
Allosteric regulation Alphavirus Viral Nonstructural Proteins Virus Replication Intrinsically disordered proteins Article 03 medical and health sciences Virology Protein Interaction Mapping Humans Binding site Cells Cultured Adaptor Proteins Signal Transducing 030304 developmental biology 0303 health sciences Binding Sites biology Tumor Suppressor Proteins Point mutation 030302 biochemistry & molecular biology Nuclear Proteins virus diseases Fibroblasts biology.organism_classification Cell biology Cytoskeletal Proteins Viral replication Interaction with host Host-Pathogen Interactions Chikungunya virus Function (biology) Protein Binding |
Zdroj: | Virology |
ISSN: | 0042-6822 |
Popis: | Alphavirus nsP3 proteins contains long, intrinsically disordered, hypervariable domains, HVD, which serve as hubs for interaction with many cellular proteins. Here, we have deciphered the mechanism and function of HVD interaction with host factors in alphavirus replication. Using NMR spectroscopy, we show that CHIKV HVD contains two SH3 domain-binding sites. Using an innovative chemical shift perturbation signature approach, we demonstrate that CD2AP interaction with HVD is mediated by its SH3-A and SH3-C domains, and this leaves the SH3-B domain available for interaction with other cellular factor(s). This cooperative interaction with two SH3 domains increases binding affinity to CD2AP and possibly induces long-range allosteric affects in HVD. Our data demonstrate that BIN1, CD2AP and SH3KBP1 play redundant roles in initiation of CHIKV replication. Point mutations in both CHIKV HVD binding sites abolish its interaction with all three proteins, CD2AP, BIN1 and SH3KBP1. This results in strong inhibition of viral replication initiation. |
Databáze: | OpenAIRE |
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