Physical training reverses defect in 3-ketoacid CoA-transferase activity in skeletal muscle of diabetic rats
| Autor: | Gilles Tancrède, André Nadeau, Adil El Midaoui, Jean Louis Chiasson |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment Ketone Bodies Fatty Acids Nonesterified Statistics Nonparametric Diabetes Mellitus Experimental Random Allocation Gastrocnemius muscle Physical Conditioning Animal Physiology (medical) Internal medicine Diabetes mellitus medicine Animals Insulin 3-Hydroxybutyric Acid Rats Wistar Treadmill Muscle Skeletal business.industry Body Weight Skeletal muscle Streptozotocin medicine.disease Rats Endocrinology medicine.anatomical_structure Ketone bodies Coenzyme A-Transferases business medicine.drug |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 288:E748-E752 |
| ISSN: | 1522-1555 0193-1849 |
| Popis: | To investigate one potential mechanism whereby physical training improves the plasma concentration of ketone bodies in experimental diabetes mellitus, we measured the activity of 3-ketoacid CoA-transferase, the key enzyme in the peripheral utilization of ketone bodies. Diabetes was induced with streptozotocin (50 mg/kg) and training carried out on a treadmill with a progressive 10-wk program. Diabetes resulted in an increase ( P < 0.001) in plasma concentration of β-hydroxybutyric acid in sedentary rats, which was partly reversed by training ( P < 0.001). Diabetes was also associated with a decreased activity of 3-ketoacid CoA-transferase in gastrocnemius muscle. When expressed per total gastrocnemius, training increased the activity of 3-ketoacid CoA-transferase by 66% in nondiabetic rats ( P < 0.001) and by 150% in diabetic rats ( P < 0.001), the decrease present in diabetic rats being fully reversed by training. Simple linear regression between the log of 3-ketoacid CoA-transferase activity and the log of plasma β-hydroxybutyric acid levels showed a statistically significant ( r = 0.563, P < 0.001) negative correlation. The beneficial effects of training on plasma ketone bodies in diabetic rats are probably explained, at least in part, by an increase in ketone body utilization, mediated by an increase in skeletal muscle 3-ketoacid CoA-transferase activity. |
| Databáze: | OpenAIRE |
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