Liquid chromatographic assay of dityrosine in human cerebrospinal fluid
| Autor: | Stephen L. Facchina, Jeannean Carver, Alison J. White, Elena Morris, Maged Abdelrahim, Ajay Verma |
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| Rok vydání: | 1997 |
| Předmět: |
Detection limit
Chromatography Resolution (mass spectrometry) Chemistry Extraction (chemistry) Reproducibility of Results General Chemistry Sensitivity and Specificity Fluorescence High-performance liquid chromatography Mass Spectrometry Fluorescence spectroscopy Spectrometry Fluorescence Cerebrospinal fluid Humans Tyrosine Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 696:175-182 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(97)00248-x |
| Popis: | A micro-scale method for separation and measurement of dityrosine in human cerebrospinal fluid (CSF) is described utilizing liquid-liquid extraction and ion-paired, reversed-phase high-performance liquid chromatography with fluorimetric detection. A mobile phase containing 1-heptanesulfonic acid linearly increased in methanol from 0 to 100% over 30 min allows the resolution of dityrosine from other fluorescent compounds with excitation at 285 nm and emission at 410 nm. As little as 0.15 ml CSF sample can be utilized with a detection limit of 60 pg dityrosine on the column. This method facilitates the use of CSF dityrosine as a measure of free radical mediated protein damage in the central nervous system. |
| Databáze: | OpenAIRE |
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