Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay
| Autor: | Anna E. V. Hagström, Sagar Dhamane, Gavin Garvey, Ulrich Strych, Richard C. Willson, Mary K. Estes, Robert L. Atmar, Andrew S. Paterson, Meena Adhikari, Katerina Kourentzi |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
lcsh:Medicine
medicine.disease_cause Diagnostic tools bacteriophage antigen binding genetics Bacteriophages lcsh:Science Bacteria (microorganisms) Caliciviridae Infections Multidisciplinary limit of detection Infectious dose nanoparticle 3. Good health unclassified drug virology Gastroenteritis Norwalk virus bioassay virus like agent Biological Assay Research Article Biology Sensitivity and Specificity Short incubation period Article Microbiology medicine Escherichia coli Humans controlled study diagnostic test accuracy study human procedures intermethod comparison virus detection Detection limit phage nanoparticle reporter nonhuman Extramural isolation and purification Norovirus lcsh:R lateral flow assay Multiple modes assay Virology United States enzyme linked immunosorbent assay High resistance 7 INGENIERÍA Y TECNOLOGÍA Nanoparticles lcsh:Q metabolism gold nanoparticle |
| Zdroj: | PLoS ONE, Vol 10, Iss 5, p e0126571 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundredfold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair. © 2015 Hagström et al. |
| Databáze: | OpenAIRE |
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