The Response to Postnatal Stress: Amino Acids Transporters and PKC Activity
| Autor: | Carla Rodríguez, Adrián Emanuel Salatino, Mariano José Scolari, Gabriela Beatriz Acosta, María Mercedes Odeon |
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| Rok vydání: | 2010 |
| Předmět: |
Male
GABA Plasma Membrane Transport Proteins medicine.medical_specialty Otras Ciencias Biológicas In Vitro Techniques Biology Biochemistry Ciencias Biológicas GABA Cellular and Molecular Neuroscience Stress Physiological Internal medicine Neuroplasticity Serine medicine Animals Staurosporine PKC Rats Wistar Protein Kinase C Protein kinase C Cerebral Cortex chemistry.chemical_classification Transporter General Medicine In vitro Rats Amino acid Cold Temperature L-SERINA Kinetics Endocrinology medicine.anatomical_structure ACUTE COLD STRESS chemistry Cerebral cortex Amino Acid Transport Systems Basic Corticosterone CIENCIAS NATURALES Y EXACTAS Intracellular Synaptosomes medicine.drug |
| Zdroj: | Neurochemical Research. 35:967-975 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-010-0153-z |
| Popis: | During postnatal development, the central nervous system (CNS) is highly sensitive to effects of drugs, stressors and environment. In this study, we evaluated the effect of acute cold stress on g-aminobutyric acid (GABA) and L-serine (L-Ser) transporters in vitro, using the uptake of [3H]GABA and [3H]L-Ser by synaptosomes-enriched fractions isolated from rat cerebral cortex during postnatal development. We found that the maximal velocity (Vmax) L-Ser uptake after stress session was increased in almost all ages studied, except at postnatal-days (PD) 5, whereas the values of Km (uptake affinity) decreased in all ages from 5 to 21 PD compared with the control group. The highest values of Vmax and Km were obtained at PD7. At the same time, in GABA uptake, the Km decreased although Vmax increased in all ages considered after cold stress during development. Finally, we investigated the mechanism by which cells regulate the substrate affinity of L-serine and GABA transporters. We demonstrated a significantly increase in PKC activity to PD5 from PD21. Pretreatment with PKC inhibitor: staurosporine (SP) led to a restoration of control uptake in several postnatal-days suggesting a relationship between amino acids uptake and PKC activation. These findings suggest that a single exposure to postnatal cold stress at different periods after birth modifies both GABA and L-Ser transporters and the related increase in PKC activity could be intracellular events that participate in neuronal plasticity by early-life stress, which could be relevant to function of transporters in the adult rat brain. Fil: Odeon, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Salatino, Adrián Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Rodríguez, Carla Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Scolari, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Acosta, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina |
| Databáze: | OpenAIRE |
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