Alterations in Ca2+ cycling proteins and G alpha q signaling after left ventricular assist device support in failing human hearts
| Autor: | Darryl L. Kirkpatrick, William T. Abraham, Richard A. Walsh, Thunder Jalili, Brian D. Hoit, Yasuchika Takeishi, Lynne E. Wagoner |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Physiology medicine.medical_treatment Immunoblotting Calcium-Transporting ATPases Biology Statistics Nonparametric GTP-Binding Proteins Physiology (medical) Internal medicine medicine Humans Protein kinase A RGS2 Protein kinase C Protein Kinase C Heart Failure Phospholipase C Myocardium Calcium-Binding Proteins Middle Aged medicine.disease Phospholamban Isoenzymes Sarcoplasmic Reticulum Endocrinology Gq alpha subunit Ventricular assist device Heart failure Case-Control Studies biology.protein Female Heart-Assist Devices Cardiology and Cardiovascular Medicine RGS Proteins Signal Transduction |
| Zdroj: | Cardiovascular research. 45(4) |
| ISSN: | 0008-6363 |
| Popis: | Objective: Left ventricular assist device support mechanically unloads the failing ventricle with resultant improvement in cardiac geometry and function in patients with end-stage heart failure. Activation of the Gαq signaling pathway, including protein kinase C, appears to be involved in the progression of heart failure. Similarly down-regulation of Ca2+ cycling proteins may contribute to contractile depression in this clinical syndrome. Thus we examined whether protein kinase C activation and decreased Ca2+ cycling protein levels could be reversed by left ventricular assist device support. Methods: Left ventricular myocardial specimens were obtained from seven patients during placement of left ventricular assist device and heart transplantation. We examined changes in protein levels of Gαq, phospholipase C β1, regulators of G protein signaling (RGS), sarcoplasmic reticulum Ca2+ ATPase, phospholamban and translocation of protein kinase C isoforms (α, β1, and β2). Results: The paired pre- and post- left ventricular assist device samples revealed that RGS2, a selective inhibitor of Gαq, was decreased ( P |
| Databáze: | OpenAIRE |
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