Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates
| Autor: | Mehdi Zeghal, Guillaume Tresset, Prabal K. Maiti, Didier Law-Hine, Sylvain Prévost, Stéphane Bressanelli, Virginie Bailleux, Anil Kumar Sahoo, Doru Constantin |
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| Přispěvatelé: | Laboratoire de Physique des Solides (LPS), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11), Department of Physics, Indian Institute of Science, European Synchrotron Radiation Facility (ESRF), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-0039,PALM,Physics: Atoms, Light, Matter(2010), Laboratoire de Physique des Solides (UMR CNRS 8502 - Université Paris-Sud) ( LPS ), European Synchrotron Radiation Facility ( ESRF ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Sud - Paris 11 ( UP11 ), ANR-10-LABX-0039-PALM,LabEx PALM,Laboratoire d’Excellence Physics Atoms Light Mater, Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
Icosahedral symmetry
[SDV]Life Sciences [q-bio] kinetic pathway law.invention Capsid X-Ray Diffraction Ab initio quantum chemistry methods law Icosahedral viral capsid Scattering Small Angle RNA Viruses General Materials Science Physical and Theoretical Chemistry [ SDV ] Life Sciences [q-bio] Chemistry Physics Virion Global fitting modeling disassembly Synchrotron Virus Crystallography Biophysics time-resolved small-angle X-ray scattering Spatiotemporal resolution |
| Zdroj: | Journal of Physical Chemistry Letters Journal of Physical Chemistry Letters, American Chemical Society, 2015, 6 (17), pp.3471-6. ⟨10.1021/acs.jpclett.5b01478⟩ Journal of Physical Chemistry Letters, American Chemical Society (ACS), 2015, 6 (17), pp.3471-6. 〈10.1021/acs.jpclett.5b01478〉 Journal of Physical Chemistry Letters, 2015, 6 (17), pp.3471-6. ⟨10.1021/acs.jpclett.5b01478⟩ |
| ISSN: | 1948-7185 |
| DOI: | 10.1021/acs.jpclett.5b01478 |
| Popis: | International audience; Viral capsids derived from an icosahedral plant virus widely used in physical and nanotechnological investigations were fully dissociated into dimers by a rapid change of pH. The process was probed in vitro at high spatiotemporal resolution by time-resolved small-angle X-ray scattering using a high brilliance synchrotron source. A powerful custom-made global fitting algorithm allowed us to reconstruct the most likely pathway parametrized by a set of stoichiometric coefficients and to determine the shape of two successive intermediates by ab initio calculations. None of these two unexpected intermediates was previously identified in self-assembly experiments, which suggests that the disassembly pathway is not a mirror image of the assembly pathway. These findings shed new light on the mechanisms and the reversibility of the assembly/disassembly of natural and synthetic virus-based systems. They also demonstrate that both the structure and dynamics of an increasing number of intermediate species become accessible to experiments. |
| Databáze: | OpenAIRE |
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