Expression of Tumor-Associated Differentially Expressed Gene-14 (TADG-14/KLK8) and Its Protein hK8 in Uterine Endometria and Endometrial Carcinomas
| Autor: | Nobutaka Nagai, Lijun Gu, Timothy J. O'Brien, Hong Hua Jin, Koso Ohama, Yoshiki Kudo, Mayu Yunokawa, Hirotoshi Tanimoto, Kazushi Shigemasa |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Gene Expression Endometrial Carcinomas Biology Endometrium Internal medicine Biomarkers Tumor medicine Carcinoma Humans TUMOR-ASSOCIATED DIFFERENTIALLY EXPRESSED GENE 14 RNA Messenger Menstrual Cycle Messenger RNA Sex Steroid Hormones Reverse Transcriptase Polymerase Chain Reaction General Medicine Middle Aged medicine.disease Immunohistochemistry Endometrial Neoplasms Secretory phase Endocrinology Biomarker (medicine) Female Kallikreins |
| Zdroj: | Tumor Biology. 27:274-282 |
| ISSN: | 1423-0380 1010-4283 |
| Popis: | To clarify the biological behavior of TADG-14/KLK8, we investigated TADG-14/KLK8 mRNA by semiquantitative RT-PCR and hK8 expression by immunohistochemistry using 37 normal endometria and 44 endometrial carcinoma tissues. TADG-14/KLK8 mRNA expression levels were significantly higher in proliferative compared to secretory phase endometria (p = 0.0143). Levels of TADG-14/KLK8 mRNA expression correlated with hK8 protein levels. hK8 was detected in 73.3% (11/15) of endometria with a significantly higher detection rate in the proliferative compared to secretory and atrophic phase endometria (p = 0.0002). High expression of hK8 was found in 61.4% of endometrial carcinomas compared to 35.1% of endometrial tissue samples (p = 0.0187). hK8 expression was significantly higher in stage I (p = 0.0433, 0.0038) and grade 1/2 (G1/2) of the tumors (p = 0.0195, 0.0044). We suggest that expression of TADG-14/KLK8may be regulated by sex steroid hormones in endometria. Our results indicate that elevated TADG-14/KLK8 expression is an early event in endometrial carcinogenesis, and may potentially serve as a useful early biomarker for the detection of endometrial carcinomas in menopausal women. |
| Databáze: | OpenAIRE |
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