Chemical Composition and Biological Activity of Essential Oil and Extract from the Seeds of Tropaeolum majus L. var. altum
| Autor: | Ivana Vrca, Dina Ramić, Željana Fredotović, Sonja Smole Možina, Ivica Blažević, Tea Bilušić |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
General Chemical Engineering
dragoljub benzil-izotiocijanat benzil-cijanid antiproliferativna aktivnost antimikrobna aktivnost Tropaeolum majus L Benzyl isothiocyanate Benzyl cyanide Antiproliferative activity Antimicrobial activity Industrial and Manufacturing Engineering benzyl isothiocyanate benzyl cyanide antiproliferative activity antimicrobial activity Food Science Biotechnology |
| Zdroj: | Food Technology and Biotechnology Volume 60 Issue 4 |
| ISSN: | 1334-2606 1330-9862 |
| Popis: | Research background. Plant Tropaeolum majus L. (garden nasturtium) belongs to the family Tropaeolaceae and contains benzyl glucosinolate. The breakdown product of benzyl glucosinolate, benzyl isothiocyanate (BITC), exhibits various biological activities such as antiproliferative, antibacterial and antiinflammatory. In order to optimize the content of biologically active volatile compounds in plant extract and essential oil, the use of appropriate extraction technique has a crucial role. Experimental approach. The current study investigates the effect of two modern extraction methods, microwave-assisted distillation (MAD) and microwave hydrodiffusion and gravity (MHG), on the chemical composition of volatile components present in the essential oil and extract of garden nasturtium (T. majus L. var. altum) seeds. Investigation of the biological activity of samples (essential oil, extract and pure compounds) was focused on the antiproliferative effect against different cancer cell lines: cervical cancer cell line (HeLa), human colon cancer cell line (HCT116) and human osteosarcoma cell line (U2OS), and the antibacterial activity which was evaluated against the growth and adhesion of Staphylococcus aureus and Escherichia coli to polystyrene surface. Results and conclusions. Essential oil and extract of garden nasturtium (T. majus) seeds were isolated by two extraction techniques: MAD and MHG. BITC and benzyl cyanide (BCN) present in the extract were identified by gas chromatography-mass spectrometry. Essential oil of T. majus showed higher antiproliferative activity (IC50 Pozadina istraživanja. Biljka dragoljub (Tropaeolum majus L.) pripada porodici Tropaeolaceae i sadržava benzil-glukozinolat. Razgradni produkt benzil-glukozinolata, benzil-izotiocijanat (BITC), ispoljava različite biološke aktivnosti, poput antiproliferativne, antibakterijske i protuupalne. U optimiranju sadržaja biološki aktivnih, hlapljivih spojeva u biljnom ekstraktu i eteričnom ulju ključnu ulogu ima primjena odgovarajuće tehnike ekstrakcije. Eksperimentalni pristup. U ovom je istraživanju ispitan učinak dviju naprednih tehnika ekstrakcije, destilacije potpomognute mikrovalovima (MAD) i mikrovalne hidrodifuzije i gravitacije (MHG), na kemijski sastav hlapljivih komponenti prisutnih u eteričnom ulju i ekstraktu sjemenki visokog dragoljuba (T. majus L. var. altum). Istraživanje biološke aktivnosti uzoraka (eteričnog ulja, ekstrakta i čistih spojeva) bilo je usmjereno na njihov antiproliferativni učinak na stanične linije raka grlića maternice (HeLa), raka debelog crijeva (HCT116) i osteosarkoma (U2OS), te na njihov učinak na suzbijanje rasta i adhezije bakterija Staphylococcus aureus i Escherichia coli na polistirenskoj površini. Rezultati i zaključci. Eterično ulje i ekstrakt sjemenki dragoljuba (T. majus) izolirani su dvjema tehnikama ekstrakcije, MAD i MHG. Prisutnost benzil-izotiocijanata i benzil-cijanida utvrđena je plinskom kromatografijom spregnutom s masenom spektrometrijom. Eterično ulje dragoljuba imalo je izraženiji antiproliferativni učinak (IC50 |
| Databáze: | OpenAIRE |
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