Effects of different hormonal treatments on growth parameters and secondary metabolite production in organ culture of Hyssopus officinalis L
| Autor: | Hengameh Shishavani, Hanieh Mohajjel Shoja |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
fungi
food and beverages Plant Science Photosynthetic pigment growth parameters secondary metabolite Secondary metabolite Biology Organ culture thidiazuron chemistry.chemical_compound chemistry Thidiazuron Botany medicine Hyssopus officinalis TP248.13-248.65 Hormone medicine.drug hyssopus officinalis photosynthetic pigment Biotechnology |
| Zdroj: | BioTechnologia, Vol 102, Iss 1, Pp 33-41 (2021) |
| ISSN: | 2353-9461 0860-7796 |
| Popis: | Hyssopus officinalis L. is a medicinal plant containing valuable phytochemicals and is used extensively in the pharma¬ceutical industry. As this plant naturally contains low levels of these metabolites, plant cell and tissue cul¬ture technologies are used to produce them in large volumes. The present study aimed first to evaluate the effects of different types of cytokinins, including benzyl adenine (BA), kinetin (KIN), and thidiazuron (TDZ), at 1 ppm concentration in the culture medium on growth parameters and production of secondary metabolites and photo¬synthetic pigments in organ culture of H. officinalis; in the second step, the study investigated the effect of dif¬ferent concentrations (0, 0.2, 1, 1.5, and 2 ppm) of the most effective type of cytokinin. The results showed that TDZ was the optimal type of growth regulator compared to BA and KIN as it showed a positive influence on the studied traits; furthermore, its highest concentration of 2 ppm in culture medium was determined to be the best one that enabled the highest production of secondary metabolites and photosynthetic pigments. We conclude that the presence of growth regulators can strongly influence the growth and development of plants in tissue culture conditions. |
| Databáze: | OpenAIRE |
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