Point-of-care multiplexed diagnosis of meningitis using the FilmArray® ME panel technology

Autor: Laetitia Ninove, Didier Raoult, Christian Kandil, Michel Drancourt, Pierre-Yves Levy, Christine Zandotti, Jean-Jacques Vincent, Sophie Alexandra Baron
Přispěvatelé: Fédération de Bactériologie-Virologie-Hygiène [AP-HM Hôpital La Timone], Hôpital de la Timone [CHU - APHM] (TIMONE), Unité des Virus Emergents (UVE), Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM), Aix-Marseille Université - Faculté de médecine (AMU MED), Aix Marseille Université (AMU), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Rok vydání: 2020
Předmět:
Adult
Male
0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
Microbiological culture
FilmArray
030106 microbiology
Pcr assay
Sensitivity and Specificity
Rapid detection
Cohort Studies
03 medical and health sciences
0302 clinical medicine
Medical microbiology
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Internal medicine
medicine
Humans
Meningitis
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Prospective Studies
030212 general & internal medicine
Child
Enterovirus
Point of care
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
business.industry
General Medicine
medicine.disease
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Predictive value
3. Good health
Infectious Diseases
Point-of-Care Testing
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Encephalitis
Female
Molecular diagnosis
business
Multiplex Polymerase Chain Reaction
Zdroj: European Journal of Clinical Microbiology and Infectious Diseases
European Journal of Clinical Microbiology and Infectious Diseases, Springer Verlag, 2020, ⟨10.1007/s10096-020-03859-y⟩
European Journal of Clinical Microbiology and Infectious Diseases, 2020, ⟨10.1007/s10096-020-03859-y⟩
ISSN: 1435-4373
0934-9723
Popis: International audience; Infectious meningitis is a medical urgency and rapid detection of the causative pathogen into the cerebrospinal fluid (CSF) is mandatory to guide the management of patients. We compared the performances of the multiplexed PCR FilmArray® ME panel with standard microbiological analyses, for rapid diagnosis of infectious meningitis. All the CSF samples received in our routine laboratory for the diagnosis of infectious meningitis were prospectively analyzed by the FilmArray® ME panel for the detection of fourteen targets in parallel to standard routine real-time PCR assays and bacterial culture. We reviewed clinical and biological records of patients for whom a discrepant result was obtained to achieve a definite diagnosis. Among 1124 CSF samples tested over a 43-week period, 113 (10.1%) and 87 (7.74%) were positive using the FilmArray® ME panel and the standard techniques, respectively. Among 40 CSF samples which yielded discrepant results, 34 were positive only using the FilmArray® ME panel and 6 were positive only using standard techniques. A total of 16/34 (47.1%) FilmArray® ME panel-positive CSF, and 6/6 (100%) of standard technique-positive CSF were interpreted as true positive. We were able to estimate the sensitivity, the specificity, the positive predictive value, and the negative predictive value of the FilmArray® ME panel at 94.2%, 98.2%, 84.3%, and 99.4%, respectively. The FilmArray® ME panel is an efficient tool for the rapid diagnosis of infectious meningitis at the point-of-care. Its higher sensitivity compared with that of standard molecular biology and culture techniques yields an increase of true positive diagnosis.
Databáze: OpenAIRE
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