Two residues in the hemagglutinin of A/Fujian/411/02-like influenza viruses are responsible for antigenic drift from A/Panama/2007/99
| Autor: | Winnie Chan, Hui Liu, Hong Jin, Kutubuddin Mahmood, Helen Zhou, Min-Shi Lee, George Kemble, Lopa Adhikary |
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| Rok vydání: | 2005 |
| Předmět: |
Models
Molecular Antigenic drift A/Panama/2007/1999 and A/Fujian/411/02-like H3N2 strains Genes Viral Protein Conformation viruses Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Biology medicine.disease_cause complex mixtures Virus Microbiology Neutralization Tests Virology parasitic diseases Influenza A virus medicine Antigenic variation Panama Hemagglutination assay food and beverages virus diseases Hemagglutination Inhibition Tests Antigenic Variation Influenza vaccines Viral replication Amino Acid Substitution biology.protein |
| Zdroj: | Virology. 336(1) |
| ISSN: | 0042-6822 |
| Popis: | The H3N2 vaccine strain (A/Panama/2007/99) for the 2003–2004 influenza season did not antigenically match the circulating A/Fujian/411/02-like H3N2 viruses and had reduced effectiveness against influenza outbreaks. A/Wyoming/03/2003, an A/Fujian-like virus, was recommended as the vaccine strain for the 2004–2005 season. A/Wyoming differed from A/Panama by 16 amino acids in the HA1 molecule. Reverse genetics was used to determine the minimal amino acid changes that were responsible for the antigenic drift from A/Panama to A/Wyoming. After substitutions of 2 of the 16 amino acids in the HA (H155T, Q156H), the A/Panama HA variant was antigenically equivalent to A/Wyoming as determined by hemagglutination inhibition and microneutralization assays using ferret postinfection antisera. Conversely, A/Wyoming containing the His-155 and Gln-156 residues from A/Panama was antigenically equivalent to A/Panama. These results indicated that only these two HA residues specified the antigenic drift from A/Panama to A/Wyoming; other amino acid differences between these two H3N2 viruses had minimal impact on virus antigenicity but impacted virus replication efficiency in eggs. |
| Databáze: | OpenAIRE |
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