Social and immunological differences among uninfected Brazilians exposed or unexposed to human immunodeficiency virus-infected partners
| Autor: | Rafaela Grossi Oliveira e Silva, Dirceu Bartolomeu Greco, Agdemir Waleria Aleixo, Lúcia Fernandes Aleixo, Laís Alves Ferreira, Marcelo Antônio Pascoal-Xavier, Willian Cunha Domingos, Victor Hugo Melo, Maria Luiza Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Male lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Sexual Behavior RC955-962 lcsh:QR1-502 Enzyme-Linked Immunosorbent Assay HIV Infections Lymphocyte Activation Microbiology HIV Serosorting Monocytes Statistics Nonparametric immune activation lcsh:Microbiology Immune system Immunophenotyping immunophenotyping Immunity Arctic medicine. Tropical medicine HIV Seronegativity Surveys and Questionnaires BDNA test Medicine Cytotoxic T cell Humans Heterosexuality business.industry flow cytometry Coitus CD28 HIV-1 discordant couples Articles Natural killer T cell Virology QR1-502 Killer Cells Natural Sexual Partners Socioeconomic Factors Immunology HIV-1 Natural Killer T-Cells RNA Viral Female business CD8 Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 109, Iss 6, Pp 775-781 (2014) Memórias do Instituto Oswaldo Cruz, Volume: 109, Issue: 6, Pages: 775-781, Published: 09 SEP 2014 Memórias do Instituto Oswaldo Cruz |
| ISSN: | 1678-8060 |
| Popis: | Understanding the social conditions and immunological characteristics that allow some human immunodeficiency virus (HIV)-exposed patients to remain uninfected represents an on-going challenge. In this study, the socio-demographic and sexual behaviour characteristics and immune activation profiles of uninfected individuals exposed to HIV-infected partners were investigated. A confidential and detailed questionnaire was administered and venous blood was tested using HIV-1/enzyme immunoassays, plasma HIV-1 RNA levels/bDNA and immunophenotyping/flow cytometry to determine the frequencies of CD4 and CD8 T cells expressing activation markers. The data analysis showed significant differences (p < 0.05) for immune parameters in individuals who were uninfected, albeit exposed to HIV-infected partners, compared with unexposed individuals. In particular, the exposed, uninfected individuals had a higher frequency (median, minimum-maximum) of CD4+HLA-DR+ (4.2, 1.8-6.1), CD8+HLA-DR+ (4.6, 0.9-13.7), CD4+CD45RO+ (27.5, 14.2-46.6), CD4+CD45RO+CD62L+ (46.7, 33.9-67.1), CD8+CD45RA+HLA-DR+ (12.1, 3.4-35.8) and CD8+CD45RO+HLA-DR+ (9.0, 3.2-14.8) cells, a decreased percentage of CD8+CD28+ cells (11.7, 4.5-24.0) and a lower cell-surface expression of Fcγ-R/CD16 on monocytes (56.5, 22.0-130.0). The plasma HIV-1 RNA levels demonstrated detectable RNA virus loads in 57% of the HIV-1+ female partners. These findings demonstrate an activation profile in both CD4 and CD8 peripheral T cells from HIV-1 exposed seronegative individuals of serodiscordant couples from a referral centre in Belo Horizonte, state of Minas Gerais. |
| Databáze: | OpenAIRE |
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