Expression of Granulocyte Colony Stimulating Factor (G-CSF) and Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF) mRNA upon Stimulation with Phorbol Ester
| Autor: | Mitchell S. Abrahamsen, Sudesh Kothari, William P. Hammond, Toby Cole |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Lipopolysaccharides medicine.medical_specialty T-Lymphocytes Population Peripheral blood mononuclear cell Monocytes chemistry.chemical_compound Internal medicine Granulocyte Colony-Stimulating Factor medicine Humans RNA Messenger education Molecular Biology Protein Kinase C Protein kinase C Messenger RNA education.field_of_study Ionophores Ionomycin Granulocyte-Macrophage Colony-Stimulating Factor Drug Synergism Cell Biology Hematology Middle Aged Granulocyte colony-stimulating factor Endotoxins Granulocyte macrophage colony-stimulating factor Endocrinology Gene Expression Regulation chemistry Tetradecanoylphorbol Acetate Molecular Medicine Calcium Signal transduction Signal Transduction medicine.drug |
| Zdroj: | Blood Cells, Molecules, and Diseases. 21:192-200 |
| ISSN: | 1079-9796 |
| DOI: | 10.1006/bcmd.1995.0022 |
| Popis: | Stimulated human peripheral blood mononuclear cells (MNC) have been shown to express both G-CSF and GM-CSF, Furthermore, G-CSF is expressed by monocytes but not lymphocytes, whereas GM-CSF is expressed largely by T lymphocytes and at low levels in monocytes/macrophages, Here we present the effect of TPA (120-O-tetradecanoyl phorbol-13-acetate) on G-CSF and GM-CSF expression in stimulated human MNCs and T lymphocytes. We observed that TPA (30nM) decreased G-CSF mRNA levels in MNCs, while ionomycin increased G-CSF in a dose-dependent manner. TPA and ionomycin individually increased GM-CSF mRNA levels in T-lymphocytes and MNCs. Further, GM-CSF was induced synergistically by TPA plus ionomycin, whereas this combination markedly decreased G-CSF mRNA levels in MNCs. These data suggest at least two signaling pathway by which G-CSF and GM-CSF and GM-CSF mRNA levels are modulated in a mixed population of monocytes and T lymphocytes, namely protein kinase C (PKC) and calcium. These signals seems to act synergistically in lymphocytes to increase GM-CSF, and not G-CSF mRNA levels specifically. It would also appear these signals act on MNCs in an opposing manner to decrease G-CSF mRNA levels, indicating that activation of PKC and the calcium signaling pathway lead to a cell-type specific modulation of individual cytokines and precise regulation of granulocyte production. |
| Databáze: | OpenAIRE |
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