High-fat diet-induced obesity amplifies HSP70-2a and HSP90 expression in testicular tissue; correlation with proliferating cell nuclear antigen (PCNA)
| Autor: | Hamed Rezaei Agdam, Sana Moshari, Masoumeh Moradi-Ozarlou, Mazdak Razi, Simineh Shahmohamadlou, Amir Nomanzadeh |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty DNA repair DNA damage Diet High-Fat 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Heat shock protein Internal medicine Proliferating Cell Nuclear Antigen Testis medicine Animals HSP70 Heat-Shock Proteins HSP90 Heat-Shock Proteins Obesity General Pharmacology Toxicology and Pharmaceutics Rats Wistar Testosterone biology Leydig cell General Medicine Proliferating cell nuclear antigen Hsp70 Rats 030104 developmental biology Endocrinology medicine.anatomical_structure Gene Expression Regulation biology.protein Immunohistochemistry |
| Zdroj: | Life sciences. 279 |
| ISSN: | 1879-0631 |
| Popis: | Aims Current study was conducted to uncover the effect of high-fat diet (HFD)-induced obesity on heat shock proteins 70-2a and 90 expression levels and to investigate the network between these proteins with PCNA expression, endocrine status of testicular tissue and nucleotide backbone damages. Main methods For this purpose, 20 mature male Wistar rats were divided into two groups of control and HFD-received obese animals (n = 10/group). After 8 weeks from obesity approval, the animals were euthanized. The expression levels of Hsp70-2a, Hsp90 and PCNA were analyzed by qRT-PCR and immunohistochemical staining techniques. The Leydig cell distribution/mm2 of interstitial tissue, serum level of testosterone, testicular total antioxidant capacity (TAC), and mRNA and DNA damage were investigated. Key findings The obese (HFD-received) animals represented a remarkable (p Significance Therefore, considering massive testicular DNA damage in the obese (HFD-received) animals, we can conclude that an increased expression of Hsp70-2a and Hsp90 with no harmony with PCNA could not properly maintain the cellular DNA integrity and/or appropriately finalize the DNA repair process. |
| Databáze: | OpenAIRE |
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