Genetic Characterization of Type 2 Porcine Circovirus (PCV-2) from Pigs with Postweaning Multisystemic Wasting Syndrome in Different Geographic Regions of North America and Development of a Differential PCR-Restriction Fragment Length Polymorphism Assay To Detect and Differentiate between Infections with PCV-1 and PCV-2
| Autor: | Thomas E. Toth, Michael Gill, Fenaux Martijn, Xiang-Jin Meng, Patrick G. Halbur |
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| Rok vydání: | 2000 |
| Předmět: |
Circovirus
Microbiology (medical) Swine Molecular Sequence Data Genome Viral Polymerase Chain Reaction complex mixtures Clinical Veterinary Microbiology Capsid Genetic variation Genotype Animals Amino Acid Sequence Porcine circovirus associated disease Genetic variability Circoviridae Infections Phylogeny Swine Diseases Genetics Base Sequence biology Wasting Syndrome Genetic Variation Sequence Analysis DNA biology.organism_classification Virology Porcine circovirus North America Circoviridae Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Clinical Microbiology. 38:2494-2503 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Postweaning multisystemic wasting syndrome (PMWS) is an emerging disease in swine. Increasing evidence indicates that a variant strain of porcine circovirus (PCV), designated type 2 PCV (PCV-2), is responsible for PMWS. To determine the extent of genetic heterogeneity of PCV-2 isolates, the complete genomes of six PCV-2 isolates from different regions of North America were amplified by PCR and sequenced. Sequence and phylogenetic analyses confirmed that two distinct genotypes of PCV exist: nonpathogenic genotype PCV-1 and PMWS-associated genotype PCV-2. However, within the PCV-2 genotype, several minor branches that have been identified appear to be associated with geographic origins. The genomic sequences of two French PCV-2 isolates diverge the most from those of other PCV-2 isolates and form a distinct branch. Other minor but distinguishable branches have also been identified for a Taiwan PCV-2 isolate and two of the Canadian PCV-2 isolates. All the U.S. PCV-2 isolates are closely related, but the Canadian isolates vary, to some extent, in their genomic sequences. The data from this study indicate that although the genome of PCV-2 is generally stable among different isolates, PCV-2 isolates from different geographic regions vary in their genomic sequences. This variation may have important implications for PCV-2 diagnosis and research. On the basis of genetic analyses of available PCV strains, a universal PCR-restriction fragment length polymorphism (PCR-RFLP) assay was developed to detect and differentiate between infections with PCV-1 and PCV-2. This PCR-RFLP assay should be useful for studying the pathogenesis of PCV-2, for detecting PCV-2 infection in pigs from different geographic regions, and for screening donor pigs for use in xenotransplantation. |
| Databáze: | OpenAIRE |
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