Prevalence of nasal carriers of methicillin-resistant Staphylococcus aureus in primary health care units in Brazil

Autor: Lizziane Kretli Winkelstroter Eller, Lalessa de Oliveira Santos, Ana Julia Turra, Talyssa Fernandes Silva de Oliveira Rodrigues, Isabella Carolina Rodrigues dos Santos Goes, Michel Ulloffo do Nascimento, Andressa Cortes Cavalleri, Luiza Pinheiro-Hubinger, Letícia Calixto Romero, Maria Angélica Gotardi, Jéssica Cristina das Dores, Valéria Cataneli Pereira
Přispěvatelé: Univ Oeste Paulista, Universidade Estadual Paulista (Unesp), Inst Lauro Souza Lima
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
Cross-transmission
RC955-962
medicine.disease_cause
Infectious Disease Transmission
Professional-to-Patient
0302 clinical medicine
Levofloxacin
Arctic medicine. Tropical medicine
Epidemiology
Prevalence
Family Health Strategy
Prospective Studies
Cross Infection
Biofilm
Middle Aged
Staphylococcal Infections
Methicillin-resistant
Anti-Bacterial Agents
Staphylococcus aureus
Carrier State
Original Article
Female
Brazil
medicine.drug
Adult
Methicillin-Resistant Staphylococcus aureus
medicine.medical_specialty
Infectious Disease Transmission
Patient-to-Professional
Health Personnel
030231 tropical medicine
Operon ica
SCCmec
Microbial Sensitivity Tests
Nose
Microbiology
03 medical and health sciences
medicine
Humans
Healthcare workers
Cefoxitin
Primary Health Care
business.industry
Clindamycin
biochemical phenomena
metabolism
and nutrition
Methicillin-resistant Staphylococcus aureus
Carriage
Cross-Sectional Studies
Genes
Bacterial
Biofilms
business
Zdroj: Revista do Instituto de Medicina Tropical de São Paulo, Volume: 63, Article number: e14, Published: 01 MAR 2021
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e14
Revista do Instituto de Medicina Tropical de São Paulo; v. 63 (2021); e14
Revista do Instituto de Medicina Tropical de São Paulo
Instituto de Medicina Tropical (IMT)
instacron:IMT
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021)
ISSN: 0036-4665
1678-9946
Popis: Made available in DSpace on 2021-06-25T12:39:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T14:36:34Z : No. of bitstreams: 1 S0036-46652021000100208.pdf: 315461 bytes, checksum: 46e0bb1594d398e0f052e0429b6d98da (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Nasal carriage of Staphylococcus aureus by healthcare workers is of great clinical importance as it facilitates the contamination of medical devices and cross-transmission. However, studies regarding the epidemiology and dissemination of S. aureus and Methicillin-resistant S. aureus (MRSA) within the Primary Health Care in Brazil are scarce. The current study aimed to detect and characterize S. aureus and MRSA strains from the nasal cavities of 63 healthcare working in primary health care units in order to determine the prevalence of S. aureus and MRSA, biofilm formation and resistance profile of these isolates. PCR reactions were performed for detecting mecA, icaA and ical) genes. The phenotypic antimicrobial susceptibility was assessed by the disk diffusion method and biofilm formation by the Congo Red Agar (CRA) method. The MRSA isolates were typed for the Staphylococcal Cassette Chromosome mec (SCCmec). The prevalence of nasal carriage of S. aureus was 74.6%. of which 72.3% were MRSA carrying SCCmec type I (24.4%), III (34.1%), IV (36.6%). Two (4.9%) isolates presented a non-typeable cassette by the performed technique. The antimicrobial susceptibility evaluation evidenced penicillin resistance in 66.1% of S. aureus, erythromycin resistance in 49.2%. while 37.3% were resistant to oxacillin, 28.8% to cefoxitin, 5.1% to levofloxacin and 5.1% to clindamycin. All isolates were biofilm producers and 96.6% of the strains contained the ica biofilm-forming genes (icaA and/or ical)). We have demonstrated a high prevalence of S. aureus and MRSA carriage among health care working in Primary health Care units, the presence of SCCmec types I, III and IV, in addition to their high ability to form biofilm, factors that possibly contribute to the dissemination and persistence of these pathogens within the primary care services. These observations highlight the importance of broadening the perspective of Health Care-Associated Infections prevention, including all health care levels, which are currently little explored. In addition, the dynamics and resistance mechanisms of S. aureus transmission still need to be further clarified to enable the implementation of more effective prevention measures. Univ Oeste Paulista, Rua Jose Bongiovani 700, BR-19050920 Presidente Prudente, SP, Brazil Univ Estadual Paulista, Dept Ciencias Quim & Biol, Botucatu, SP, Brazil Inst Lauro Souza Lima, Dept Patol, Bauru, SP, Brazil Univ Estadual Paulista, Dept Ciencias Quim & Biol, Botucatu, SP, Brazil FAPESP: 2017/01104-5
Databáze: OpenAIRE
Externí odkaz: