Comprehensive characterization of the patient-derived xenograft and the paralleled primary hepatocellular carcinoma cell line
| Autor: | Chit Chow, Kui Fat Chan, Linda Ng, Kwok Wai Lo, Chi Wai Yip, Siu Tim Cheung, Tan To Cheung, Phyllis F. Y. Cheung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Cancer Research Hepatocellular carcinoma Cell line establishment 03 medical and health sciences 0302 clinical medicine In vivo Cancer stem cell Patient-derived xenograft Internal medicine medicine Genetics Viability assay Primary cell business.industry medicine.disease Phenotype In vitro 030104 developmental biology Cell culture 030220 oncology & carcinogenesis business Primary Research |
| Zdroj: | Cancer Cell International |
| Popis: | Background Hepatocellular carcinoma (HCC) is an aggressive cancer with high mortality and morbidity worldwide. The limited clinically relevant model has impeded the development of effective HCC treatment strategy. Patient-derived xenograft (PDX) models retain most of the characteristics of original tumors and were shown to be highly predictive for clinical outcomes. Notably, primary cell line models allow in-depth molecular characterization and high-throughput analysis. Combined usage of the two models would provide an excellent tool for systematic study of therapeutic strategies. Here, we comprehensively characterized the novel PDX and the paralleled primary HCC cell line model. Methods Tumor tissues were collected from HCC surgical specimens. HCC cells were sorted for in vivo PDX and in vitro cell line establishment by the expression of hepatic cancer stem cell marker to enhance cell viability and the rate of success on subsequent culture. The PDX and its matching primary cell line were authenticated and characterized in vitro and in vivo. Results Among the successful cases for generating PDXs and primary cells, HCC40 is capable for both PDX and primary cell line establishment, which were then further characterized. The novel HCC40-PDX and HCC40-CL exhibited consistent phenotypic characteristics as the original tumor in terms of HBV protein and AFP expressions. In common with HCC40-PDX, HCC40-CL was tumorigenic in immunocompromised mice. The migration ability in vitro and metastatic properties in vivo echoed the clinical feature of venous infiltration. Genetic profiling by short tandem repeat analysis and p53 mutation pattern consolidated that both the HCC40-PDX and HCC40-CL models were derived from the HCC40 clinical specimen. Conclusions The paralleled establishment of PDX and primary cell line would serve as useful models in comprehensive studies for HCC pathogenesis and therapeutics development for personalized treatment. Electronic supplementary material The online version of this article (doi:10.1186/s12935-016-0322-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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