Evaluation of Recombination Repair Pathways in Thermal Radiosensitization
| Autor: | J. Maude-Leblanc, L. Li, G. P. Raaphorst |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Hot Temperature
DNA Repair Cell Survival Mutant Biophysics CHO Cells Biology Radiation Tolerance Cell Line Mice Cricetulus Cricetinae Homologous chromosome Animals Radiology Nuclear Medicine and imaging Recombination Genetic Genetics Ku70 Radiation Thermal radiosensitization Embryo Cell biology DNA-Binding Proteins Non-homologous end joining Cell culture embryonic structures Homologous recombination Chickens DNA Damage |
| Zdroj: | Radiation Research. 161:215-218 |
| ISSN: | 1938-5404 0033-7587 |
| Popis: | Raaphorst, G. P., Maude-Leblanc, J. and Li, L. Evaluation of Recombination Repair Pathways in Thermal Radiosensitization. Radiat. Res. 161, 215–218 (2004). Thermal radiosensitization has been shown to cause inhibition of repair of sublethal and potentially lethal damage and DNA DSBs. In this study we assessed thermal radiosensitization in mutants deficient in homologous recombinational (HR) repair and nonhomologous end joining repair (NHEJ). Using cells of the mouse wild-type embryo fibroblast cell line MEF and its Ku80–/– derivative that is deficient in NHEJ, we showed that thermal radiosensitization is the same in both cell lines. Further studies with cells of the wild-type CHO-AA8 cell line and its derivative IRSISF, which is deficient in HR, also showed comparable thermal radiosensitization in both cell lines. Further experiments using cells of chicken DT40 cell lines also showed comparable thermal radiosensitization between the wild-type HR mutant Rad54, the NHEJ mutant Ku70, and the double ... |
| Databáze: | OpenAIRE |
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