In Vivo Survival and Osteogenic Differentiation of Allogeneic Rat Bone Marrow Mesenchymal Stem Cells (MSCs)
| Autor: | Mika Tadokoro, Hajime Ohgushi, Noriko Kotobuki, Youichi Katou, Motohiro Hirose, Yoshihiro Katsube |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Ceramics
Allogeneic transplantation Cell Survival Biomedical Engineering lcsh:Medicine Bone Marrow Cells Biology Mesenchymal Stem Cell Transplantation Regenerative medicine Bone and Bones Tacrolimus Graft Enhancement Immunologic Implants Experimental Osteogenesis Animals Transplantation Homologous Progenitor cell Cells Cultured Stem cell transplantation for articular cartilage repair Transplantation Mesenchymal stem cell lcsh:R Cell Differentiation Mesenchymal Stem Cells Cell Biology Coculture Techniques Rats Inbred F344 Rats Endothelial stem cell Durapatite Rats Inbred Lew Cancer research Stem cell Immunosuppressive Agents |
| Zdroj: | Cell Transplantation, Vol 17 (2008) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | Marrow mesenchymal stem cells (MSCs) are multipotent progenitor cells and reported to be immunoprivileged as well as immunosuppressive. Hence, MSCs might be ideal candidates for allogeneic transplantation to induce regeneration of damaged tissues/organs. To confirm the differentiation capability of allogeneic MSCs in vivo is important for the acceleration of regenerative medicine. Consequently, we have established an in vivo rat model using subcutaneous implantation of a hydroxyapatite (HA) ceramic/MSCs composite. Osteogenic differentiation was used as an indicator of differentiation. When syngeneic MSCs were implanted, MSCs showed osteogenic differentiation as evidenced by new bone formation as well as high alkaline phosphatase (ALP) activity. When allogeneic MSCs were implanted, none of the allografts survived or showed osteogenic differentiation. However, when the recipient rats were treated with FK506 immunosuppressant, allogeneic MSCs showed osteogenic differentiation. Although this finding might not be adequate for the acceleration of regenerative medicine, these results did confirm that MSCs are not intrinsically immunoprivileged but that under appropriate immunosuppressant treatment, allogeneic MSCs can survive and show differentiation capability in vivo. |
| Databáze: | OpenAIRE |
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