3D DNA origami designed with caDNAno

Autor: George Amoako, ZhiYong Shen, Rian Ye, Ming Zhou, LiZhou Zhuang, XiaoHong Yang
Jazyk: angličtina
Předmět:
Zdroj: Chinese Science Bulletin. 58(24):3019-3022
ISSN: 1001-6538
DOI: 10.1007/s11434-013-5879-y
Popis: For about three decades, DNA-based nanotechnology has been undergoing development as an assembly method for nanostructured materials. The DNA origami method pioneered by Rothemund paved the way for the formation of 3D structures using DNA self assembly. The origami approach uses a long scaffold strand as the input for the self assembly of a few hundred staple strands into desired shapes. Herein, we present a 3D origami “roller” (75 nm in length) designed using caDNAno software. This has the potential to be used as a template to assemble nanoparticles into different pre-defined shapes. The “roller” was characterized with agarose gel electrophoresis, atomic force microscopy (AFM) and transmission electron microscopy (TEM).
Databáze: OpenAIRE