Changes of buoyant density during the S-phase of the cell cycle. Direct evidence demonstrated in acute myeloid leukemia by flowcytometry
| Autor: | E. Modderman, W. Huiges, Simon Daenen, M. R. Halie |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cancer Research
Direct evidence Cell Buoyant density S Phase Flow cytometry BUOYANT DENSITY Myeloblast medicine Humans CELL CYCLE S phase medicine.diagnostic_test FLOW-CYTOMETRIC Chemistry Myeloid leukemia DNA Hematology Cell cycle Flow Cytometry Cell biology SPECIFIC GRAVITY Leukemia Myeloid Acute medicine.anatomical_structure Oncology Immunology MYELOBLAST ACUTE MYELOID LEUKEMIA |
| Zdroj: | Leukemia Research, 17(1), 37-41. PERGAMON-ELSEVIER SCIENCE LTD |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/0145-2126(93)90139-c |
| Popis: | Studies with synchronized or exponentially growing bacteria and mammalian cell lines are not able to demonstrate small changes in buoyant density during the cell cycle. Flowcytometric analysis of density separated acute myeloid leukemia cells, a system not dependent on time-related variables, shows that the cellular buoyant density increases slightly with up to 0.008 g/ml during the S-phase, at least in cryo-preserved cells used in this study. This contrasts with the generally accepted belief that S-phase cells have a lower or constant buoyant density. A practical implication is that separation of cell (sub)populations based on differences in buoyant density could be flawed to the extent that these populations contain S-phase cells. |
| Databáze: | OpenAIRE |
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